Conservation Genetics Resources

, Volume 8, Issue 3, pp 259–261 | Cite as

An environmental DNA marker for detecting nonnative brown trout (Salmo trutta)

  • K. J. CarimEmail author
  • T. M. Wilcox
  • M. Anderson
  • D. J. Lawrence
  • M. K. Young
  • K. S. McKelvey
  • M. K. Schwartz
Technical Note


Brown trout (Salmo trutta) are widely introduced in western North America where their presence has led to declines of several native species. To assist conservation efforts aimed at early detection and eradication of this species, we developed a quantitative PCR marker to detect the presence of brown trout DNA in environmental samples. The marker strongly amplified brown trout eDNA, and produced no amplification of eDNA from 17 other species commonly found in western North America. We field tested this marker and demonstrated positive detections in field samples where brown trout presence was known.


Salmonids eDNA Invasive species qPCR 

Supplementary material

12686_2016_548_MOESM1_ESM.docx (24 kb)
Supplementary material 1 (DOCX 24 kb)


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Copyright information

© Springer Science+Business Media Dordrecht (outside the USA) 2016

Authors and Affiliations

  • K. J. Carim
    • 1
    Email author
  • T. M. Wilcox
    • 1
    • 2
  • M. Anderson
    • 3
  • D. J. Lawrence
    • 4
  • M. K. Young
    • 1
  • K. S. McKelvey
    • 1
  • M. K. Schwartz
    • 1
  1. 1.Rocky Mountain Research Station, United States Department of Agriculture, Forest ServiceNational Genomics Center for Wildlife and Fish ConservationMissoulaUSA
  2. 2.Division of Biological SciencesUniversity of MontanaMissoulaUSA
  3. 3.Arizona Department of Game and FishPhoenixUSA
  4. 4.National Fish and Wildlife FoundationWashingtonUSA

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