Using environmental DNA to detect an endangered crayfish Cambaroides japonicus in streams
- 695 Downloads
The freshwater crayfish, Cambaroides japonicus, is endangered in Hokkaido, Japan and inhabits burrows. Here, we applied environmental DNA (eDNA) method for evaluating the species distributions with comparing hand-capture method in the headwater streams. We detected the eDNA of C. japonicus from all sites, where we collected C. japonicus, and confirmed that eDNA can be applied to detect burrowing aquatic freshwater crayfish without disturbing their habitats.
KeywordsSpecies-specific primers Real-time PCR Lotic system Species detection
We thank to Takashi Mitsuzuka, and Atsuko Kato for their assistances for the sampling. We wish to appreciate Itsuro Koizumi, Toru Suzuki, Toshifumi Minamoto, Teruhiko Takahara, Hitoshi Araki, Masanori Nunokawa, and Ryota Kawanishi, who kindly provide their comments on this study.
- Kawai T, Nakata K, Hamano T (2002) Temporal changes of the density in two crayfish species, the native Cambaroides japonicus (De Haan) and the alien Pacifastacus leniusculus (Dana), in natural habitats of Hokkaido, Japan. Freshw Crayfish 13:198–206Google Scholar
- R Core Team (2015) R: a language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. https://www.R-project.org/