Conservation Genetics Resources

, Volume 8, Issue 3, pp 201–206 | Cite as

Development of SNP and microsatellite markers for goldsinny wrasse (Ctenolabrus rupestris) from ddRAD sequencing data

  • E. Jansson
  • J. B. Taggart
  • S. Wehner
  • G. Dahle
  • M. Quintela
  • S. Mortensen
  • B. O. Kvamme
  • K. A. Glover
Technical Note

Abstract

Wrasse (Labridae) species have been used as parasite cleaners in Atlantic salmon farming since the 1980s. However, their use has recently escalated, with millions now being introduced into salmon cages each year. Most fish are of wild origin, their exploitation potentially impacting native populations. Genetic information is urgently required to inform management decisions. We identified 174 microsatellite and 149 SNP markers from ddRAD sequence data. From these, 17 and 48 microsatellite and SNP markers, respectively, were validated by genotyping 150 goldsinny wrasse collected from five locations along the Norwegian and Swedish coasts. Two to 30 alleles were identified at the microsatellite loci, while gene diversity (He) ranged 0.101–0.907. All SNP loci were biallelic, with averaged He per locus ranging between 0.063 and 0.495.

Keywords

SNP Microsatellite Goldsinny wrasse Ctenolabrus rupestris Cleaner fish 

Notes

Acknowledgments

This study was financed by the Norwegian Department for Trade and Fisheries (NFD). The Swedish Cultural Foundation in Finland (Svenska kulturfonden) is acknowledged for personal grant to EJ. The authors also acknowledge the support of the MASTS pooling initiative (The Marine Alliance for Science and Technology for Scotland). The authors thank Paulo Prodohl for supplying biotinylated oligos.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

12686_2016_532_MOESM1_ESM.xlsx (77 kb)
Supplementary material 1–4 (XLSX 77 kb)

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Copyright information

© Springer Science+Business Media Dordrecht 2016

Authors and Affiliations

  • E. Jansson
    • 1
  • J. B. Taggart
    • 3
  • S. Wehner
    • 3
  • G. Dahle
    • 1
  • M. Quintela
    • 1
  • S. Mortensen
    • 1
  • B. O. Kvamme
    • 1
  • K. A. Glover
    • 1
    • 2
  1. 1.Institute of Marine ResearchBergenNorway
  2. 2.Department of Biology, Sea Lice Research CentreUniversity of BergenBergenNorway
  3. 3.Institute of Aquaculture, School of Natural SciencesUniversity of StirlingStirlingUK

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