A novel multiplex PCR assay for individual identification in the vulnerable Chinese Egret (Egretta eulophotes) using molted feathers
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Noninvasive DNA may be used in individual identification for counting or tracking animals. Here a multiplex PCR assay, in which 16 highly polymorphic microsatellite markers were chosen and allocated into four multiplex PCR panels, was described for the individual identification of the Chinese Egret (Egretta eulophotes) using molted feathers. In this study, the combined exclusion probability for unrelated individuals (PID) and for individuals with sibling relationship (PID(sib)) were 7.02 × 10−19 and 2.9 × 10−7, respectively. Molted feathers (n = 59) of E. eulophotes amplified by more than 7 loci were unambiguously identified as 43 different individuals (32 females and 11 males). The results indicated that our newly developed assay could be used for noninvasive individual identification in the vulnerable Chinese Egret and other Ardeids.
KeywordsMultiplex PCR Microsatellite genotyping Individual identification Noninvasive DNA Egret
We thank Wei Lei, Site Luo, Leiguan Li and Changfu Mao who helped collect some samples for this study. This research was supported by the National Natural Science Foundation of China (Grant Nos. 41476113, 31000963 and 31272333) and by the Fujian Natural Science Foundation of China (2010Y2007 and 2012J01149).
- IUCN (2014) IUCN red list of threatened species. http://www.iucnredlist.org/. Accessed 7 October 2014
- Rudnick JA, Katzner TE, Bragin EA, Rhodes OE, Dewoody JA (2005) Using naturally shed feathers for individual identification, genetic parentage analyses, and population monitoring in an endangered Eastern imperial eagle (Aquila heliaca) population from Kazakhstan. Mol Ecol 14:2959–2967. doi: 10.1111/j.1365-294X.2005.02641.x CrossRefPubMedGoogle Scholar