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Conservation Genetics Resources

, Volume 7, Issue 3, pp 743–749 | Cite as

Development and characterization of 16 microsatellite markers for Micromeria (Lamiaceae) from Tenerife (Canary Islands, Spain) using 454 sequencing

  • Pamela Puppo
  • Manuel Curto
  • Harald Meimberg
Microsatellite Letters

Abstract

Micromeria presents eight species in Tenerife, and from these, three are endangered or critically endangered. In this paper we use a 454 run from M. hyssopifolia to develop and characterize 16 new microsatellite markers and test them using 19 individuals from two populations of this species. The number of alleles per locus varied from 2 to 10 and observed heterozygosity ranged from 0.125 to 0.900. Most loci successfully amplified in the threatened species M. densiflora, M. glomerata and M. rivas-martinezii and were also polymorphic. These loci will be valuable tools for assessing the genetic diversity of these endangered species.

Keywords

Lamiaceae Micromeria Microsatellites 454 GS-FLX 

Notes

Acknowledgments

The University of La Laguna in Tenerife provided a vehicle for the field trips. P.L. Pérez de Paz (ULL) was an invaluable help during collection of the plant material. This research was supported by the Fundação para a Ciência e Tecnologia (FCT) with a research Grant to H.M. (PTDC/BIA-BEC/108866/2008) and PhD fellowships to P.P. (SFRH/BD/74747/2010) and M.C. (SFRH/BD/79010/2011).

Supplementary material

12686_2015_451_MOESM1_ESM.doc (32 kb)
Supplementary material 1 (DOC 31 kb)

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Copyright information

© Springer Science+Business Media Dordrecht 2015

Authors and Affiliations

  1. 1.CIBIO, Research Center in Biodiversity and Genetic Resources, InBio Associate LaboratoryUniversity of PortoVairãoPortugal
  2. 2.Institute for Integrative Nature Conservation ResearchUniversity of Natural Resources and Life SciencesViennaAustria

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