Advertisement

Conservation Genetics Resources

, Volume 7, Issue 2, pp 569–572 | Cite as

Highly polymorphic microsatellite markers in two species, the invasive shore crabs Hemigrapsus sanguineus and Hemigrapsus takanoi (Decapoda, Varunidae)

  • Céline Poux
  • Moâna Gothland
  • Anne-Catherine Holl
  • Nicolas Spilmont
  • Jean-François Arnaud
Microsatellite Letters

Abstract

Varunid crabs Hemigrapsus sanguineus and Hemigrapsus takanoi are species native from intertidal coastal areas in North-western Pacific and have become invasive along the Atlantic European coasts. To gain insights into population genetic features, we developed and characterized 16 (H. sanguineus) and 11 (H. takanoi) novel polymorphic microsatellite loci from next-generation sequencing. The number of alleles ranged from 2 to 20 for H. sanguineus, and from 8 to 21 for H. takanoi. Expected heterozygosity ranged from 0.470 to 0.947 and from 0.313 to 0.781, with mean multilocus F is estimates suggesting rapid turnover of populations. Overall, these microsatellite markers showed very high levels of polymorphism that will facilitate population genetic studies devoted to track down the most likely sources of introduction.

Keywords

Hemigrapsus Biological invasion Intertidal coastal areas Microsatellites 

Notes

Acknowledgments

This work received financial support from the French program “EC2CO”.

Supplementary material

12686_2015_426_MOESM1_ESM.doc (28 kb)
Supplementary material 1 (DOC 27 kb)
12686_2015_426_MOESM2_ESM.doc (26 kb)
Supplementary material 2 (DOC 26 kb)

References

  1. Asakura A, Watanabe S (2005) Hemigrapsus takanoi, new species, a sibling species of the common Japanese intertidal crab H. penicillatus (Decapoda: Brachyura: Grapsoidea). J Crust Biol 25:279–292CrossRefGoogle Scholar
  2. Favre-Bac L, Godé C, Arnaud J-F (2014) Characterization of polymorphic microsatellite markers for the fine-leaved water-dropwort Oenanthe aquatica and the Gypsywort Lycopus europaeus, two farmland remnant wetland species. Conserv Genet Res 6:995–998CrossRefGoogle Scholar
  3. Gothland M, Dauvin J-C, Denis L, Dufossé F, Jobert S, Ovaert J, Pezy J-P, Tous Rius A, Spilmont N (2014) Biological traits explain the distribution and colonisation ability of the invasive shore crab Hemigrapsus takanoi. Estuar Costal Shelf Sci 142:41–49CrossRefGoogle Scholar
  4. Goudet J (1995) FSTAT (Version 1.2). A computer program to calculate F-statistics. J Hered 86:485–486Google Scholar
  5. Malausa T, Gilles A, Meglécz E, Blanquart H, Duthoy S, Costedoat C, Dubut V, Pech N, Castagnone-Sereno P, Délye C, Feau N, Frey P, Gauthier P, Guillemaud T, Hazard L, Le Corre V, Lung-Escarmant B, Malé P-J, Ferreira S, Martin J-F (2011) High-throughput microsatellite isolation through 454 GS-FLX titanium pyrosequencing of enriched DNA libraries. Mol Ecol Res 11:638–644CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2015

Authors and Affiliations

  • Céline Poux
    • 1
  • Moâna Gothland
    • 2
  • Anne-Catherine Holl
    • 1
  • Nicolas Spilmont
    • 2
    • 3
  • Jean-François Arnaud
    • 1
  1. 1.Unité Évolution, Écologie, Paléontologie, UMR CNRS 8198Université Lille 1 – Sciences et TechnologiesVilleneuve d’Ascq CedexFrance
  2. 2.Laboratoire d’Océanologie et de Géosciences, UMR CNRS 8187Université Lille 1 – Sciences et TechnologiesWimereuxFrance
  3. 3.Environmental Futures Research InstituteGrifith UniversityGold CoastAustralia

Personalised recommendations