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Conservation Genetics Resources

, Volume 7, Issue 2, pp 557–559 | Cite as

Development and characterization of polymorphic microsatellite markers for the crested caracara, Caracara cheriway

  • Erin E. Vaughn
  • James F. Dwyer
  • Joan L. Morrison
  • Melanie Culver
Microsatellite Letters
  • 85 Downloads

Abstract

We isolated novel microsatellites from the crested caracara (Caracara cheriway) with a shotgun pyrosequencing approach. We tested 80 loci for polymorphism among 20 individuals from the threatened Florida population. Fourteen loci were polymorphic. The mean number of alleles was 2.21 (range 2–3) and the mean observed heterozygosity was 0.41 (range 0.15–0.65). None of the 14 polymorphic loci exhibited significant linkage disequilibrium nor did they deviate significantly from Hardy–Weinberg expectations. We also report 16 monomorphic loci.

Keywords

Microsatellite Falconidae Florida 454 Pyrosequencing 

Notes

Acknowledgments

We would like to thank Debbie Chen and Mila Crevar for their assistance in primer testing. Any use of trade, firm, or product names is for descriptive purpose only and does not imply endorsement by the US Government.

Supplementary material

12686_2015_423_MOESM1_ESM.doc (110 kb)
Supplementary material 1 (DOC 109 kb)

References

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Copyright information

© Springer Science+Business Media Dordrecht 2015

Authors and Affiliations

  • Erin E. Vaughn
    • 1
    • 2
  • James F. Dwyer
    • 5
  • Joan L. Morrison
    • 3
  • Melanie Culver
    • 2
    • 4
  1. 1.Genetics Graduate Interdisciplinary ProgramUniversity of ArizonaTucsonUSA
  2. 2.Conservation Genetics Laboratory, School of Natural Resources and EnvironmentUniversity of ArizonaTucsonUSA
  3. 3.Department of BiologyTrinity CollegeHartfordUSA
  4. 4.US Geological SurveyArizona Cooperative Fish and Wildlife Research UnitTucsonUSA
  5. 5.EDM International, Inc.Fort CollinsUSA

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