Conservation Genetics Resources

, Volume 7, Issue 2, pp 557–559 | Cite as

Development and characterization of polymorphic microsatellite markers for the crested caracara, Caracara cheriway

  • Erin E. Vaughn
  • James F. Dwyer
  • Joan L. Morrison
  • Melanie Culver
Microsatellite Letters


We isolated novel microsatellites from the crested caracara (Caracara cheriway) with a shotgun pyrosequencing approach. We tested 80 loci for polymorphism among 20 individuals from the threatened Florida population. Fourteen loci were polymorphic. The mean number of alleles was 2.21 (range 2–3) and the mean observed heterozygosity was 0.41 (range 0.15–0.65). None of the 14 polymorphic loci exhibited significant linkage disequilibrium nor did they deviate significantly from Hardy–Weinberg expectations. We also report 16 monomorphic loci.


Microsatellite Falconidae Florida 454 Pyrosequencing 



We would like to thank Debbie Chen and Mila Crevar for their assistance in primer testing. Any use of trade, firm, or product names is for descriptive purpose only and does not imply endorsement by the US Government.

Supplementary material

12686_2015_423_MOESM1_ESM.doc (110 kb)
Supplementary material 1 (DOC 109 kb)


  1. Meglécz E, Costedoat C, Dubut V, Gilles A, Malausa T, Pech N, Martin J (2010) QDD: a user friendly program to select microsatellite markers and design primers from large sequencing projects. Bioinformatics 26(3):403–404CrossRefPubMedGoogle Scholar
  2. Morrison JL, Dwyer JF (2012) Crested caracara (Caracara cheriway), the birds of North America online In: Poole A (ed). Ithaca: Cornell Lab of Ornithology; retrieved from the birds of North America online:
  3. Morrison JL, Humphrey SR (2001) Conservation value of private lands for crested caracaras in Florida. Conserv Biol 15:675–684CrossRefGoogle Scholar
  4. Rousset F (2008) GENEPOP′007: a complete re-implementation of the GENEPOP software for Windows and Linux. Mol Ecol Resour 8(1):103–106CrossRefPubMedGoogle Scholar
  5. Van Oosterhout C, Hutchinson WF, Wills DPM, Shipley P (2004) MICRO-CHECKER: software for identifying and correcting genotyping errors in microsatellite data. Mol Ecol Notes 4:535–538CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2015

Authors and Affiliations

  • Erin E. Vaughn
    • 1
    • 2
  • James F. Dwyer
    • 5
  • Joan L. Morrison
    • 3
  • Melanie Culver
    • 2
    • 4
  1. 1.Genetics Graduate Interdisciplinary ProgramUniversity of ArizonaTucsonUSA
  2. 2.Conservation Genetics Laboratory, School of Natural Resources and EnvironmentUniversity of ArizonaTucsonUSA
  3. 3.Department of BiologyTrinity CollegeHartfordUSA
  4. 4.US Geological SurveyArizona Cooperative Fish and Wildlife Research UnitTucsonUSA
  5. 5.EDM International, Inc.Fort CollinsUSA

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