Development and characterization of polymorphic microsatellite markers for the crested caracara, Caracara cheriway
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We isolated novel microsatellites from the crested caracara (Caracara cheriway) with a shotgun pyrosequencing approach. We tested 80 loci for polymorphism among 20 individuals from the threatened Florida population. Fourteen loci were polymorphic. The mean number of alleles was 2.21 (range 2–3) and the mean observed heterozygosity was 0.41 (range 0.15–0.65). None of the 14 polymorphic loci exhibited significant linkage disequilibrium nor did they deviate significantly from Hardy–Weinberg expectations. We also report 16 monomorphic loci.
KeywordsMicrosatellite Falconidae Florida 454 Pyrosequencing
We would like to thank Debbie Chen and Mila Crevar for their assistance in primer testing. Any use of trade, firm, or product names is for descriptive purpose only and does not imply endorsement by the US Government.
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