Conservation Genetics Resources

, Volume 7, Issue 2, pp 521–524 | Cite as

Development and characterization of microsatellite markers from the humivorous termite Cavitermes tuberosus (Isoptera: Termitinae) using pyrosequencing technology

Microsatellite Letters

Abstract

Eighteen microsatellite markers from the humivorous termite Cavitermes tuberosus (Isoptera: Termitidae: Termitinae) were developed using a procedure of microsatellite-enriched libraries pyrosequencing. They were optimized in four multiplex PCR sets, and tested on 38 individuals collected in French Guiana. The number of alleles per locus ranged from 3 to 13. The expected and observed heterozygosities varied from 0.279 to 0.867 and from 0.237 to 0.789, respectively. Cross-species amplifications of these loci were performed in eight other neotropical species of Termitidae. These new microsatellite markers will constitute useful tools to study population genetics, reproductive strategies and dispersal patterns of C. tuberosus, which is a common representative of the humivorous guild, fulfilling an essential function in soil nutrient recycling.

Keywords

Cavitermes tuberosus Termite Microsatellite Pyrosequencing Population genetics 

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Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  1. 1.Evolutionary Biology and Ecology, CP 160/12Université libre de BruxellesBrusselsBelgium
  2. 2.Chemistry of Social Insects, Institute of Organic Chemistry and BiochemistryAcademy of Sciences of the Czech RepublicPragueCzech Republic

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