Isolation and characterization of polymorphic microsatellite markers from a globally distributed marine apex predator, the tiger shark (Galeocerdo cuvier)
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High levels of fishing have resulted in declines among many of the oceans top predators, including the globally distributed tiger shark (Galeocerdo cuvier). Overexploitation of this species has led to declines in parts of its distribution, which may have important ecological consequences given the tiger shark’s trophic position as a large bodied, generalist predator. To assess the population genetics of this species, an enrichment protocol was used to isolate a suite of nine di- and tetra-nucleotide microsatellite loci within the tiger shark, which were subsequently characterized using genomic DNA derived from 40 individuals sampled from Hawaiian waters. The number of alleles per locus ranged from 3 to 23, and the average expected heterozygosities across loci ranged from 0.30 to 0.93. Analyses suggested a low frequency of null alleles across markers, and all loci conformed to both Hardy–Weinberg and linkage equilibrium at P > 0.01.
KeywordsTiger shark Galeocerdo cuvier Microsatellite Elasmobranch genetics
Funding provided by grants from the Save Our Seas Foundation and Guy Harvey Ocean Foundation (MSS), Pritzker Foundation to the Pritzker Laboratory for Molecular Systematics and Evolution (KAF), and a Natural Sciences and Engineering Research Council of Canada (NSERC) Postgraduate Scholarship (AMB). We thank T. Daly-Engel, K. Duncan, D. Grubbs, and B. Wetherbee for samples.
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