Development and characterization of novel microsatellite markers for Arion slug species
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Seventeen polymorphic microsatellite markers were isolated and characterized in Arion vulgaris/lusitanicus, which belongs to the worst European slug pests with serious economic and ecological impact. These markers were tested on 23 individuals collected in a population in Switzerland. Numbers of alleles ranged from 2 to 14 per locus, observed and expected heterozygosities ranged from 0.174 to 0.87, and from 0.162 to 0.903, respectively. These loci were also successfully amplified and were polymorphic in the closely related species A. rufus and A. ater. These loci represent the first highly polymorphic nuclear markers described for A. vulgaris and pave the way for population genetics and molecular ecology research of the important Arion pest slugs.
KeywordsArion vulgaris Arion lusitanicus Arion rufus Arion ater 454 Pyrosequencing Invasive species Mollusks
We thank Susanne Tellenbach for help in the laboratory, and the Next Generation Sequencing Platform and Doreen Becker at the University of Bern, Institute of Genetics, for assistance and access to infrastructure. This project has been supported by the Swiss Federal Office for the Environment (BAFU).
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