Identification of 246 microsatellites in the Asiatic clam (Corbicula fluminea)
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Asiatic clam (Corbicula fluminea) is one of the most invasive freshwater bivalves. Despite the ecologic and economic impacts of this species, there are only a few polymorphic microsatellites. In this study, we screened the genome from C. fluminea in search of microsatellite markers using massive parallel sequencing. We identified 246 new microsatellites involving di-, tri-, tetra- and pentanucleotide single repeats and different compound microsatellites, and we used a validation protocol to characterize a sample of those. Nine of them presented two or more alleles indicating a low level of variability.
KeywordsAsiatic clam Corbicula fluminea Microsatellites Massively parallel sequencing
We are indebted to Gisela Mir from CRAG Laboratory for her help in obtaining MPS data. The authors thank Cesca Rivas for assistance with laboratory procedures. This research was carried out within the objectives of the Research Project CGL200909407 of the Spanish MICINN.
- Peñarrubia L, Sanz N, Pla C, Vidal O, Viñas J (2014) Using Massive Parallel Sequencing for population genetics markers development, validation and application in the invasive bivalve zebra mussel (Dreissena polymorpha)Google Scholar