Advertisement

Conservation Genetics Resources

, Volume 7, Issue 2, pp 393–395 | Cite as

Identification of 246 microsatellites in the Asiatic clam (Corbicula fluminea)

  • Luis Peñarrubia
  • Rosa-Maria Araguas
  • Carles Pla
  • Nuria Sanz
  • Jordi Viñas
  • Oriol VidalEmail author
Microsatellite Letters

Abstract

Asiatic clam (Corbicula fluminea) is one of the most invasive freshwater bivalves. Despite the ecologic and economic impacts of this species, there are only a few polymorphic microsatellites. In this study, we screened the genome from C. fluminea in search of microsatellite markers using massive parallel sequencing. We identified 246 new microsatellites involving di-, tri-, tetra- and pentanucleotide single repeats and different compound microsatellites, and we used a validation protocol to characterize a sample of those. Nine of them presented two or more alleles indicating a low level of variability.

Keywords

Asiatic clam Corbicula fluminea Microsatellites Massively parallel sequencing 

Notes

Acknowledgments

We are indebted to Gisela Mir from CRAG Laboratory for her help in obtaining MPS data. The authors thank Cesca Rivas for assistance with laboratory procedures. This research was carried out within the objectives of the Research Project CGL200909407 of the Spanish MICINN.

Supplementary material

12686_2014_378_MOESM1_ESM.doc (209 kb)
Supplementary material 1 (DOC 209 kb)
12686_2014_378_MOESM2_ESM.doc (200 kb)
Supplementary material 2 (DOC 199 kb)

References

  1. Benson G (1999) Tandem repeats finder: a program to analyze DNA sequences. Nucleic Acids Res 27:573–580. doi: 10.1093/nar/27.2.573 CrossRefPubMedCentralPubMedGoogle Scholar
  2. Botstein D, White RL, Skolnick M, Davis RW (1980) Construction of a genetic linkage map in man using restriction fragment length polymorphisms. Am J Hum Genet 32:314–331PubMedCentralPubMedGoogle Scholar
  3. Peñarrubia L, Sanz N, Pla C, Vidal O, Viñas J (2014) Using Massive Parallel Sequencing for population genetics markers development, validation and application in the invasive bivalve zebra mussel (Dreissena polymorpha)Google Scholar
  4. Pigneur LM, Risterucci AM, Dauchot N, Li X, Van DK (2011) Development of novel microsatellite markers to identify the different invasive lineages in the Corbicula complex and to assess androgenesis. Mol Ecol Resour 11:573–577. doi: 10.1111/j.1755-0998.2010.02963.x CrossRefPubMedGoogle Scholar
  5. Schuelke M (2000) An economic method for the fluorescent labeling of PCR fragments. Nat Biotechnol 18:233–234. doi: 10.1038/72708 CrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • Luis Peñarrubia
    • 1
  • Rosa-Maria Araguas
    • 1
  • Carles Pla
    • 1
  • Nuria Sanz
    • 1
  • Jordi Viñas
    • 1
  • Oriol Vidal
    • 1
    Email author
  1. 1.Laboratori d’Ictiologia Genètica, Departament de BiologiaUniversitat de GironaGironaSpain

Personalised recommendations