Advertisement

Conservation Genetics Resources

, Volume 7, Issue 1, pp 57–59 | Cite as

A novel primer set for mammal species identification from feces samples

  • Florencia Grattarola
  • S. González
  • M. CosseEmail author
Technical Note

Abstract

We described a technique for detecting mammal species, based on the analysis of a control region fragment of mitochondrial DNA by establishing taxonomic identity from non-invasive samples. We detected a polymorphic fragment that varies in sequence and length within different mammalian species but maintains its identity among individuals of the same species. We amplified a single fragment in all the mammalian species tested from tissue samples and identified feces samples at species level. The use of a unique set of primers to assess the presence of different mammal species with non-invasive sampling allowed us to differentiate sequences from more than one species per environmental sample. Thus, it constitutes a powerful molecular tool for inventory and description of the mammal diversity distribution in natural areas.

Keywords

Noninvasive sampling Species determination Mammalia monitoring Wildlife forensics 

References

  1. Bozarth C, Alva-Campbell Y, Ralls K, Henry T, Smith D, Westphal M, Maldonado J (2010) An efficient noninvasive method for discriminating among faeces of sympatric North American canids. Conserv Genet Resour 2:173–175Google Scholar
  2. González S, Duarte JMB (2007) Noninvasive methods for genetic analysis applied to ecological and behavioral studies in Latino-America. Rev Bras Zootec 36:89–92CrossRefGoogle Scholar
  3. Hausknecht R, Gula R, Pirga B, Kuehn R (2007) Urine—a source for noninvasive genetic monitoring in wildlife. Mol Ecol Notes 7:208–212CrossRefGoogle Scholar
  4. Kohn MH, Wayne RK (1997) Facts from feces revisited. Trends Ecol Evol 12:223–227CrossRefPubMedGoogle Scholar
  5. Medrano JF, Aasen E, Sharrow L (1990) DNA extraction from nucleated red blood cells. Biotechniques 8:43Google Scholar
  6. Nowak C, Büntjen M, Steyer K, Frosch C (2014) Testing mitochondrial markers for noninvasive genetic species identification in European mammals. Conserv Genet Resour 6:41–44CrossRefGoogle Scholar
  7. Pun K-M, Albrecht C, Castella V, Fumagalli L (2009) Species identification in mammals from mixed biological samples based on mitochondrial DNA control region length polymorphism. Electrophoresis 30:1008–1014CrossRefPubMedGoogle Scholar
  8. Schwartz MK, Luikart G, Waples RS (2007) Genetic monitoring as a promising tool for conservation and management. Trends Ecol Evol 22:25–33CrossRefPubMedGoogle Scholar
  9. Sheppard SK, Harwood JD (2005) Advances in molecular ecology: tracking trophic links through predator-prey food-webs. Funct Ecol 19:751–762CrossRefGoogle Scholar
  10. Symondson WOC (2002) Molecular identification of prey in predator diets. Mol Ecol 11:627–641CrossRefPubMedGoogle Scholar
  11. Taberlet P, Gordon L (1999) Non-invasive genetic sampling and individual identification. Biol J Linn Soc 68:41–55CrossRefGoogle Scholar
  12. Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739CrossRefPubMedCentralPubMedGoogle Scholar
  13. Vila C, Amorim IR, Leonard JA, Posada D, Castroviejo J, Petrucci-Fonseca F, Crandall KA, Ellegren H, Wayne RK (1999) Mitochondrial DNA phylogeography and population history of the grey wolf Canis lupus. Mol Ecol 8:2089–2103CrossRefPubMedGoogle Scholar
  14. Waits LP, Paetkau D (2005) Noninvasive genetic sampling tools for wildlife biologist: a review of applications and recommendations for accurate data collection. J Wildl Manag 69:1419–1433CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  1. 1.Genética de la Conservación-Departamento de Biodiversidad y GenéticaIIBCE- MECMontevideoUruguay
  2. 2.Sección Genética Evolutiva Facultad de CienciasUdelaRMontevideoUruguay

Personalised recommendations