Conservation Genetics Resources

, Volume 7, Issue 1, pp 57–59 | Cite as

A novel primer set for mammal species identification from feces samples

  • Florencia Grattarola
  • S. González
  • M. CosseEmail author
Technical Note


We described a technique for detecting mammal species, based on the analysis of a control region fragment of mitochondrial DNA by establishing taxonomic identity from non-invasive samples. We detected a polymorphic fragment that varies in sequence and length within different mammalian species but maintains its identity among individuals of the same species. We amplified a single fragment in all the mammalian species tested from tissue samples and identified feces samples at species level. The use of a unique set of primers to assess the presence of different mammal species with non-invasive sampling allowed us to differentiate sequences from more than one species per environmental sample. Thus, it constitutes a powerful molecular tool for inventory and description of the mammal diversity distribution in natural areas.


Noninvasive sampling Species determination Mammalia monitoring Wildlife forensics 


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Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  1. 1.Genética de la Conservación-Departamento de Biodiversidad y GenéticaIIBCE- MECMontevideoUruguay
  2. 2.Sección Genética Evolutiva Facultad de CienciasUdelaRMontevideoUruguay

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