Conservation Genetics Resources

, Volume 7, Issue 1, pp 263–267 | Cite as

Development of 27 novel cross-species microsatellite markers for the endangered Hucho bleekeri using next-generation sequencing technology

  • Ke Wang
  • Shuhuan Zhang
  • Dengqiang Wang
  • Miaomiao Xin
  • Jinming Wu
  • Qingliang Sun
  • Hao Du
  • Chengyou Wang
  • Jun Huang
  • Qiwei Wei
Microsatellite Letters
First Online:
Received:
Accepted:

Abstract

In this study, we developed 27 polymorphic microsatellite loci in Hucho bleekeri, a glacial relict and freshwater resident salmonid fish in China. The number of alleles varied from 2 to 8 for each primer set. The observed and expected heterozygosity ranged from 0.250 to 0.906 and 0.508 to 0.845, respectively. The polymorphic information content ranged from 0.371 to 0.808. These microsatellite loci should be useful to study population genetics, paternity identification, speciation and adaptive evolution of this lineage.

Keywords

Hucho bleekeri Microsatellite Endangered species 
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Notes

Acknowledgments

This work was supported by Special Fund for Agro-Scientific Research in the Public Interest (201203086) and open projects for Key Laboratory of Freshwater Biodiversity Conservation, Ministry of Agriculture of China (No. LFBC0802). The fundamental Research Funds for the Central Public Research Institutes (2013JBFM19 and 2014A07XK05). The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper.

References

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Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • Ke Wang
    • 1
  • Shuhuan Zhang
    • 2
  • Dengqiang Wang
    • 2
  • Miaomiao Xin
    • 2
  • Jinming Wu
    • 2
  • Qingliang Sun
    • 2
  • Hao Du
    • 2
  • Chengyou Wang
    • 2
  • Jun Huang
    • 2
  • Qiwei Wei
    • 1
    • 2
  1. 1.Wuxi Fisheries CollegeNanjing Agricultural UniversityWuxiChina
  2. 2.Yangtze River Fisheries Research InstituteChinese Academy of Fishery SciencesWuhanChina

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