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Conservation Genetics Resources

, Volume 7, Issue 1, pp 73–75 | Cite as

Isolation and characterization of fifteen microsatellite loci for the giant clam Tridacna maxima

  • Daphné Grulois
  • Josina Tiavouane
  • Pascal Paul Dumas
  • Cécile Fauvelot
Microsatellite Letters

Abstract

Fifteen polymorphic microsatellite markers were developed for Tridacna maxima in order to assess self-recruitment and larval dispersal within and among MPAs in New Caledonia and provide baseline data for conservation management of this species. Number of alleles varied from 5 to 25 per locus, observed and expected heterozygosities ranged from 0.171 to 0.860 and 0.591 to 0.935 respectively. Significant deviations from HWE were detected in eight loci. Cross-amplifications were tested in five other species of Tridacnidae (T. crocea, T. squamosa, T. derasa, T. noae and Hippopus hippopus).

Keywords

Tridacna crocea Tridacna noae Tridacna squamosa Microsatellite loci Marine Protected Area Paternity analyses 

Notes

Acknowledgments

This work was cofunded by the BeN-Co project (ZONECO program, New Caledonia), the COMPO project (ANR, n° 2010 JCJC 1701 01) and the TriMax project (LabEx CORAIL, ANR).

Supplementary material

12686_2014_290_MOESM1_ESM.txt (6 kb)
Supplementary material 1 (TXT 6 kb)

References

  1. Boneh L, Kuperus P, Van Tienderen PH (2003) Microsatellites in the bromeliads Tillandsia fasciculata and Guzmania monostachya. Mol Ecol Notes 3:302–303CrossRefGoogle Scholar
  2. Meglécz E (2007) MicroFamily: a computer program for detecting flanking region similarities among different microsatellite loci. Mol Ecol Notes 7:18–20CrossRefGoogle Scholar
  3. Raymond M, Rousset F (1995) GENEPOP (version 1.2): population genetics software for exact tests and ecumenicism. J Heredity 86:248–249. Genepop on the web, http://genepop.curtin.edu.au/index.html. Accessed 20 July 2014
  4. Van Oosterhout C, Hutchinson WF, Wills DPM, Shipley P (2004) Micro-Checker: software for identifying and correcting genotyping errors in microsatellite data. Mol Ecol Notes 4:535–538CrossRefGoogle Scholar
  5. Wells S (1996) Tridacna maxima. In: IUCN 2009. IUCN Red List of threatened species. Version 2014.1. www.iucnredlist.org. 17 July 2014

Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • Daphné Grulois
    • 1
  • Josina Tiavouane
    • 1
  • Pascal Paul Dumas
    • 2
  • Cécile Fauvelot
    • 1
  1. 1.LabEx CORAIL, UR 227 CoReUs 2Institut de Recherche pour le Développement (IRD)Noumea CedexNew Caledonia
  2. 2.UR 227 CoReUs 2, Fisheries Department of VanuatuInstitut de Recherche pour le Développement (IRD)Port-VilaVanuatu

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