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Conservation Genetics Resources

, Volume 7, Issue 1, pp 61–63 | Cite as

Development and characterization of polymorphic microsatellites markers in endangered Aquilaria malaccensis

  • Pradeep Singh
  • Himanshu Sharma
  • Akshay Nag
  • Brijmohan Singh Bhau
  • Ram Kumar Sharma
Microsatellite Letters

Abstract

Aquilaria malaccensis is a critically endangered species in Asia because of its overexploitation to obtain high valued agarwood, used in medicine, perfume, and incense production. Its genetic improvement faces serious problem due to lack of genomic resources. In this study, eighteen novel highly polymorphic microsatellite markers derived from enriched genomic libraries were characterized using 45 individuals of three populations, would be an asset to its non-existing marker recourse. Allelic variations per locus ranged from 2 to 6 with mean allele number was 3.28. The average observed and expected heterozygosity varied from 0.811 and 0.597, respectively. Reproducibility rate, polymorphic potential and ability to detect heterozygosity, novel microsatellites markers would contribute in evaluation of genetic diversity and population structure, and selection of elite germplasm for conservation of genetic resources and commercial utilization.

Keywords

Endangered Agarwood Microsatellites Genetic diversity Conservation 

Notes

Acknowledgments

The research work presented in the manuscript was funded by Department of Biotechnology (DBT) and Council of Scientific and Industrial Research (CSIR), Government of India. This is IHBT communication number 3707.

Supplementary material

12686_2014_287_MOESM1_ESM.doc (51 kb)
Supplementary material 1 (DOC 51 kb)

References

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Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • Pradeep Singh
    • 1
  • Himanshu Sharma
    • 1
  • Akshay Nag
    • 2
  • Brijmohan Singh Bhau
    • 2
  • Ram Kumar Sharma
    • 1
  1. 1.Division of BiotechnologyCSIR - Institute of Himalayan Bioresource TechnologyPalampurIndia
  2. 2.Division of Medicinal Aromatic and Economic PlantsCSIR-North-East Institute of Science and Technology (CSIR-NEIST)JorhatIndia

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