Development and characterization of polymorphic microsatellites markers in endangered Aquilaria malaccensis
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Aquilaria malaccensis is a critically endangered species in Asia because of its overexploitation to obtain high valued agarwood, used in medicine, perfume, and incense production. Its genetic improvement faces serious problem due to lack of genomic resources. In this study, eighteen novel highly polymorphic microsatellite markers derived from enriched genomic libraries were characterized using 45 individuals of three populations, would be an asset to its non-existing marker recourse. Allelic variations per locus ranged from 2 to 6 with mean allele number was 3.28. The average observed and expected heterozygosity varied from 0.811 and 0.597, respectively. Reproducibility rate, polymorphic potential and ability to detect heterozygosity, novel microsatellites markers would contribute in evaluation of genetic diversity and population structure, and selection of elite germplasm for conservation of genetic resources and commercial utilization.
KeywordsEndangered Agarwood Microsatellites Genetic diversity Conservation
The research work presented in the manuscript was funded by Department of Biotechnology (DBT) and Council of Scientific and Industrial Research (CSIR), Government of India. This is IHBT communication number 3707.
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