Development of microsatellite markers for the near threatened eagles Harpia harpyja and Morphnus guianensis using next-generation sequencing
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We isolated and characterized 11 microsatellite loci for Harpia harpyja using the IonTorrent PGM. The loci were validated in 22 Harpia harpyja and 16 Morphnus guianensis individuals. Ten of the loci were variable in H. harpyja, ranging from two to six alleles per locus. Seven of the loci were variable in M. guianensis ranging from two to six alleles per locus. Genotypes did not deviate from Hardy–Weinberg equilibrium expectation, and no linkage disequilibrium was observed. These markers have a promise to provide an important resource for genetic population studies, conservation and monitoring of these two near threatened accipitrid species.
KeywordsHarpy Eagle Crested Eagle Microsatellites Conservation genetics
Financial support for this project was provided by the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Fundação Grupo Boticário de Proteção à Natureza, Scott Neotropical Fund/Cleveland Metroparks Zoo, Fundação de Amparo à Pesquisa do Estado do Amazonas (FAPEAM), Fundação de Amparo à Pesquisa do Estado do Espírito Santo (FAPES) and the Convenio INPA/FDB/VALE S.A. Permission to collect tissue samples of was granted by SISBIO/IBAMA. This study is part of RC’s Master’s thesis in the Genetics, Conservation and Evolutionary Biology graduate program of INPA/UFAM. IPF and TH were supported by a Bolsa de Pesquisa scholarship from CNPq during this study. This is contribution N. 7 of the Brazilian Harpy Eagle Conservation Program (PCGR-Brazil).