Conservation Genetics Resources

, Volume 6, Issue 4, pp 999–1001 | Cite as

Development of microsatellite markers for the near threatened eagles Harpia harpyja and Morphnus guianensis using next-generation sequencing

  • Renan Coser
  • José Gregorio Martínez
  • Mário Nunes
  • Enedina N. de Assunção
  • Spartaco Astolfi-Filho
  • Aureo Banhos
  • Tânia M. Sanaiotti
  • Tomas Hrbek
  • Izeni Pires Farias
Microsatellite Letters

Abstract

We isolated and characterized 11 microsatellite loci for Harpia harpyja using the IonTorrent PGM. The loci were validated in 22 Harpia harpyja and 16 Morphnus guianensis individuals. Ten of the loci were variable in H. harpyja, ranging from two to six alleles per locus. Seven of the loci were variable in M. guianensis ranging from two to six alleles per locus. Genotypes did not deviate from Hardy–Weinberg equilibrium expectation, and no linkage disequilibrium was observed. These markers have a promise to provide an important resource for genetic population studies, conservation and monitoring of these two near threatened accipitrid species.

Keywords

Harpy Eagle Crested Eagle Microsatellites Conservation genetics 

References

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Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • Renan Coser
    • 1
  • José Gregorio Martínez
    • 1
  • Mário Nunes
    • 1
  • Enedina N. de Assunção
    • 2
  • Spartaco Astolfi-Filho
    • 2
  • Aureo Banhos
    • 3
  • Tânia M. Sanaiotti
    • 4
  • Tomas Hrbek
    • 1
  • Izeni Pires Farias
    • 1
  1. 1.Laboratório de Evolução e Genética Animal, Departamento de GenéticaUniversidade Federal do AmazonasManausBrazil
  2. 2.Centro de Apoio Muldisciplinar, Laboratório de Tecnologias de DNAUniversidade Federal do AmazonasManausBrazil
  3. 3.Departamento de Biologia, Centro de Ciências AgráriasUniversidade Federal do Espírito SantoAlegreBrazil
  4. 4.Coordenação em BiodiversidadeInstituto Nacional de Pesquisas da AmazôniaManausBrazil

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