Conservation Genetics Resources

, Volume 6, Issue 4, pp 983–985 | Cite as

Polymorphic microsatellite markers in the long-spined sea urchin Diadema setosum

  • Mingling Wang
  • Zhe Li
  • Monthon Ganmanee
  • James Y. Xie
  • Juan Diego Urriago
  • Jian-Wen Qiu
Microsatellite Letters
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Accepted:

Abstract

We developed twenty polymorphic microsatellite markers for the long-spined sea urchin Diadema setosum by screening microsatellite enriched library, and characterized their polymorphism using two populations of 45 individuals. The number of alleles per locus ranged from 4 to 17, and the observed and expected heterozygosity varied from 0.0714 to 1.000 and 0.6655 to 0.9321, respectively. Twelve loci conformed to the Hardy–Weinberg equilibrium after Bonferroni correction. These polymorphic microsatellite markers will facilitate studies of population genetics and connectivity in D. setosum.

Keywords

Microsatellite markers Polymorphism Sea urchin Diadema 
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Notes

Acknowledgments

This work was supported by Environment and Conservation Fund, Hong Kong (project 2011-08) and Hong Kong Baptist University (project FRG2/13-14/009).

Supplementary material

12686_2014_264_MOESM1_ESM.pdf (33 kb)
Supplementary material 1 (PDF 32 kb)

References

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Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • Mingling Wang
    • 1
    • 2
  • Zhe Li
    • 2
  • Monthon Ganmanee
    • 3
  • James Y. Xie
    • 2
  • Juan Diego Urriago
    • 2
  • Jian-Wen Qiu
    • 2
  1. 1.Institute of OceanologyChinese Academy of SciencesQingdaoPeople’s Republic of China
  2. 2.Department of BiologyHong Kong Baptist UniversityHong KongPeople’s Republic of China
  3. 3.Department of Animal Production Technology and Fisheries, Faculty of Agricultural TechnologyKing Mongkut’s Institute of Technology LadkrabangBangkokThailand

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