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Conservation Genetics Resources

, Volume 6, Issue 4, pp 903–905 | Cite as

Using next-generation sequencing methods to isolate and characterize 24 simple sequence repeat loci in mandrills (Mandrillus sphinx)

  • Laure Benoit
  • Sylvère Mboumba
  • E. Willaume
  • P. M. Kappeler
  • Marie J. E. CharpentierEmail author
Microsatellite Letters

Abstract

Mandrill is a vulnerable Old World primate living in the rain-forests of central Africa. This species is currently facing two major human encroachments: habitat destruction and bush-meat trade. The total population size remains unknown in the wild, but it is suspected to have recently declined. We developed and characterized 24 new polymorphic microsatellite markers from the next-generation sequencing data using 66 individuals from a wild population. The number of alleles per locus ranged from 2 to 9 and the observed heterozygosity from 0.12 to 0.92. Conveniently, the developed markers did not amplify human DNA avoiding cross-species contamination. These microsatellites will be especially useful for studies based on sensible DNA, including population genetics analyses to studies in behavioral ecology.

Keywords

NGS SSR Mandrillus sphinx 

Notes

Acknowledgments

This study was funded by the Deutsche Forschungsgemeinschaft (DFG, KA 1082-20-1 to MJEC and PMK) and a ‘Station d’Etudes en Ecologie Globale’ as well as a ‘Laboratoire International Associé’ (INEE-CNRS, to MJEC). We are grateful to the Centre Méditerranéen Environnement Biodiversité (LabEx CEMEB) for access to their technical facilities. We thank the CENAREST for providing research permits (authorization number: AR0003/12/MENESRSIC/CENAREST/CG/CST/CSAR). We are grateful to the field assistants of the ‘Mandrillus Project’ and to the veterinary staff (Stéphanie Bourgeois, Alix Ortega, Romain Cassaigne, Benoit Quintard) for darting assistance. We further thank the SODEPAL agents for their assistance. This is ‘Mandrillus Project’ publication number 3.

Supplementary material

12686_2014_237_MOESM1_ESM.pdf (21 kb)
Supplementary material 1 (PDF 21 kb)

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Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • Laure Benoit
    • 1
    • 2
  • Sylvère Mboumba
    • 3
  • E. Willaume
    • 4
  • P. M. Kappeler
    • 5
  • Marie J. E. Charpentier
    • 1
    Email author
  1. 1.CEFE-CNRS UMR 5175Montpellier Cedex 5France
  2. 2.CIRADMontpellier Cedex 5France
  3. 3.Université des Sciences et Techniques de MasukuFrancevilleGabon
  4. 4.SODEPAL (Société du Parc d’Exploitation de la Lékédi)-COMILOG SocietyBakoumbaGabon
  5. 5.Behavioral Ecology and Sociobiology UnitGerman Primate Center (DPZ)GöttingenGermany

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