Conservation Genetics Resources

, Volume 6, Issue 4, pp 883–884 | Cite as

Development of microsatellite markers for the critically endangered frog Telmatobufo bullocki and cross-species amplification in two related species

  • Virginia Moreno-Puig
  • Yeşerin Yildirim
  • Dianne H. Brunton
Microsatellite Letters
  • 85 Downloads

Abstract

Fifteen microsatellite markers were developed for Telmatobufo bullocki, a critically endangered frog species from Chile. Genetic diversity that was assessed in 29 individuals from one location showed 3–19 alleles per locus. These markers were then tested for cross-species amplification in two related and similarly threatened species. The markers will be used in genetic studies aiding the management and conservation of T. bullocki.

Keywords

Microsatellite loci Telmatobufo bullocki Cross-species amplification Callyptocephalellidae 

Notes

Acknowledgments

Funded by the European Association of Zoos and Aquaria and Auckland Zoo Conservation Fund. We thank to Paul B. Rainey and Heather Hendrickson for access to lab space and Claudio Correa for providing DNA samples. Thanks to Forestal Arauco S.A. for access permits and to Zoológico Nacional de Chile and Danté Fenolio for assistance with captive animal sampling. Research permit provided by Servicio Agrícola y Ganadero (Nº6977).

Supplementary material

12686_2014_231_MOESM1_ESM.doc (55 kb)
Supplementary material 1 (DOC 55 kb)

References

  1. Fenolio DB, Moreno-Puig V, Levy MG, Nuñez JJ, Lamar WW, Fabry MO, Tirado MS, Crump ML, Charrier A (2013) Status and conservation of a Gondwana legacy: Bullock’s false toad, Telmatobufo bullocki (Amphibia: Anura: Calyptocephalellidae). Herpetol Rev 44(4):583–590Google Scholar
  2. Meglécz E, Costedoat C, Dubut V, Gilles A, Malausa T, Pech N, Martin J-F (2010) QDD: a user-friendly program to select microsatellite markers and design primers from large sequencing projects. Bioinformatics 26(3):403–404. doi: 10.1093/bioinformatics/btp670 PubMedCrossRefGoogle Scholar
  3. Meirmans PG, Van Tienderen PH (2004) GENOTYPE and GENODIVE: two programs for the analysis of genetic diversity of asexual organisms. Mol Ecol Notes 4(4):792–794. doi: 10.1111/j.1471-8286.2004.00770.x CrossRefGoogle Scholar
  4. Schuelke M (2000) An economic method for the fluorescent labeling of PCR fragments. Nat Biotechnol 18(2):233–234PubMedCrossRefGoogle Scholar
  5. Van Oosterhout C, Hutchinson WF, Wills DPM, Shipley P (2004) Micro-checker: software for identifying and correcting genotyping errors in microsatellite data. Mol Ecol Notes 4(3):535–538. doi: 10.1111/j.1471-8286.2004.00684.x CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • Virginia Moreno-Puig
    • 1
  • Yeşerin Yildirim
    • 2
  • Dianne H. Brunton
    • 1
  1. 1.Ecology, Conservation and Behaviour Group, Institute of Natural and Mathematical SciencesMassey UniversityAucklandNew Zealand
  2. 2.New Zealand Institute for Advanced Study and Allan Wilson Centre for Molecular Ecology and EvolutionMassey UniversityAucklandNew Zealand

Personalised recommendations