Development of microsatellite markers for the critically endangered frog Telmatobufo bullocki and cross-species amplification in two related species
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Fifteen microsatellite markers were developed for Telmatobufo bullocki, a critically endangered frog species from Chile. Genetic diversity that was assessed in 29 individuals from one location showed 3–19 alleles per locus. These markers were then tested for cross-species amplification in two related and similarly threatened species. The markers will be used in genetic studies aiding the management and conservation of T. bullocki.
KeywordsMicrosatellite loci Telmatobufo bullocki Cross-species amplification Callyptocephalellidae
Funded by the European Association of Zoos and Aquaria and Auckland Zoo Conservation Fund. We thank to Paul B. Rainey and Heather Hendrickson for access to lab space and Claudio Correa for providing DNA samples. Thanks to Forestal Arauco S.A. for access permits and to Zoológico Nacional de Chile and Danté Fenolio for assistance with captive animal sampling. Research permit provided by Servicio Agrícola y Ganadero (Nº6977).
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