Conservation Genetics Resources

, Volume 6, Issue 4, pp 883–884 | Cite as

Development of microsatellite markers for the critically endangered frog Telmatobufo bullocki and cross-species amplification in two related species

  • Virginia Moreno-Puig
  • Yeşerin Yildirim
  • Dianne H. Brunton
Microsatellite Letters


Fifteen microsatellite markers were developed for Telmatobufo bullocki, a critically endangered frog species from Chile. Genetic diversity that was assessed in 29 individuals from one location showed 3–19 alleles per locus. These markers were then tested for cross-species amplification in two related and similarly threatened species. The markers will be used in genetic studies aiding the management and conservation of T. bullocki.


Microsatellite loci Telmatobufo bullocki Cross-species amplification Callyptocephalellidae 



Funded by the European Association of Zoos and Aquaria and Auckland Zoo Conservation Fund. We thank to Paul B. Rainey and Heather Hendrickson for access to lab space and Claudio Correa for providing DNA samples. Thanks to Forestal Arauco S.A. for access permits and to Zoológico Nacional de Chile and Danté Fenolio for assistance with captive animal sampling. Research permit provided by Servicio Agrícola y Ganadero (Nº6977).

Supplementary material

12686_2014_231_MOESM1_ESM.doc (55 kb)
Supplementary material 1 (DOC 55 kb)


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Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • Virginia Moreno-Puig
    • 1
  • Yeşerin Yildirim
    • 2
  • Dianne H. Brunton
    • 1
  1. 1.Ecology, Conservation and Behaviour Group, Institute of Natural and Mathematical SciencesMassey UniversityAucklandNew Zealand
  2. 2.New Zealand Institute for Advanced Study and Allan Wilson Centre for Molecular Ecology and EvolutionMassey UniversityAucklandNew Zealand

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