Conservation Genetics Resources

, Volume 6, Issue 3, pp 795–797 | Cite as

Isolation and characterization of polymorphic microsatellites in the Tibetan cypress Cupressus gigantea using paired-end Illumina shotgun sequencing

  • Shaoke Li
  • Zengqiang Qian
  • Yaru Fu
  • Weilie Zheng
  • Huie Li
Microsatellite Letters
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Abstract

Cupressus gigantea Cheng et L. K. Fu is an endangered conifer endemic to Tibet of China. In this study, 16 polymorphic microsatellite markers were developed using paired-end Illumina shotgun sequencing. The number of alleles varied between 3 and 18, with an average of 8.063 alleles per locus. The observed (H O) and expected heterozygosities (H E) ranged from 0.227 to 0.902 and from 0.000 to 1.000, respectively. Following the false discovery rate controlling procedure, six loci showed significant deviations from Hardy–Weinberg equilibrium, and linkage disequilibrium was only detected between a pair of loci. These informative microsatellite markers can be readily used for genetic analyses of this endangered plant.

Keywords

Cupressus gigantea Endangered species Microsatellites Next-generation sequencing (NGS) 
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Notes

Acknowledgments

This research was financially supported by the Department of Science & Technology of Tibet, China (Key Project: “Studies upon the Key Technologies for Protection and Restoration of Tibetan Forest Ecosystem”; 2011-01).

Supplementary material

12686_2014_222_MOESM1_ESM.doc (35 kb)
Supplementary material 1 (DOC 35 kb)

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Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • Shaoke Li
    • 1
    • 2
  • Zengqiang Qian
    • 3
  • Yaru Fu
    • 1
    • 2
  • Weilie Zheng
    • 1
    • 2
  • Huie Li
    • 4
  1. 1.Institute of Plateau EcologyTibet Agricultural and Animal Husbandry CollegeNyingchiPeople’s Republic of China
  2. 2.Key Laboratory of Forest Ecology in Tibet Plateau (Tibet University)Ministry of EducationNyingchiPeople’s Republic of China
  3. 3.College of Life SciencesShaanxi Normal UniversityXi’anPeople’s Republic of China
  4. 4.Department of Food ScienceTibet Agricultural and Animal Husbandry CollegeNyingchiPeople’s Republic of China

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