Advertisement

Conservation Genetics Resources

, Volume 6, Issue 3, pp 773–775 | Cite as

Microsatellite markers for the endangered shrub Myrceugenia rufa (Myrtaceae) and three closely related species

  • Fernanda Pérez
  • C. Carla Irarrázabal
  • Michel Cossio
  • Gioconda Peralta
  • Ricardo Segovia
  • Mauricio Bosshard
  • L. Felipe Hinojosa
Microsatellite Letters

Abstract

Myrceugenia rufa is an endangered shrub endemic to the coast range of central Chile, which has suffered strong degradation during recent decades. We developed nine microsatellite markers for this species and tested them on M. correifolia, M. lanceolata, and M. exsucca. Six loci amplified and were polymorphic in all species; the remaining loci were polymorphic in at least two species. The number of alleles per locus ranged from 1 to 10, the expected and observed heterozygosities ranged from 0.07 to 0.90 and 0.12–0.84, respectively. Characterized microsatellites will be useful to estimate the genetic diversity and population structure of M. rufa and its sister species.

Keywords

Coastal range Mediterranean forest Myrceugenia Rare species 

Notes

Acknowledgments

This work was supported by Fondecyt Grant 1110929.

References

  1. Hechenleitner P, Gardner MF, Thomas PI, Echeverría C, Escobar B, Brownless P, Martínez C (2005) Plantas amenazadas del centro-sur de Chile. Distribución, conservación y propagación. Universidad Austral de Chile y Jardín Botánico de Edimburgo, ChileGoogle Scholar
  2. Landrum L (1981) A monograph of the genus Myrceugenia (Myrtaceae). NY Bot Gard Flora Neotropica 29:1–132Google Scholar
  3. Murillo AJ, Ruiz E, Landrum L, Stuessy TF, Barfuss MH (2012) Phylogenetic relationships in Myrceugenia (Myrtaceae) based on plastid and nuclear DNA sequences. Mol Phylogenet Evol 62:764–776. doi: 10.1016/j.ympev.2011.11.021 CrossRefGoogle Scholar
  4. Peakall R, Smouse PE (2006) GENALEX 6: genetic analysis in excel. Population genetic software for teaching and research. Mol Ecol Notes 6:288–295. doi: 10.1111/j.1471-8286.2005.01155.x CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • Fernanda Pérez
    • 1
    • 2
  • C. Carla Irarrázabal
    • 1
  • Michel Cossio
    • 1
  • Gioconda Peralta
    • 1
  • Ricardo Segovia
    • 3
  • Mauricio Bosshard
    • 3
  • L. Felipe Hinojosa
    • 2
    • 3
  1. 1.Departamento de EcologíaPontificia Universidad Católica de ChileSantiagoChile
  2. 2.Institute of Ecology and Biodiversity (IEB)SantiagoChile
  3. 3.Departamento de Ciencias EcológicasUniversidad de ChileSantiagoChile

Personalised recommendations