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Conservation Genetics Resources

, Volume 6, Issue 3, pp 763–764 | Cite as

Isolation and characterisation of microsatellite markers for Mylothris jacksoni knutsoni (Lepidoptera: Pieridae), an endemic butterfly of the Gulf of Guinea Highlands

  • Jan ZimaJr.
  • Dan Lestina
  • Petr Jansta
  • Vojtech Petru
  • Robert Tropek
Microsatellite Letters

Abstract

Eight polymorphic microsatellite loci were developed and characterized for the Mylothris jacksoni knutsoni, an endemic butterfly of endangered montane habitats of the Gulf of Guinea Highlands, West/Central Africa. The loci were tested for polymorphism in 30 individuals from the Nkogam Massif, western Cameroon. The detected numbers of alleles per locus ranged from 2 to 5, and the observed and expected heterozygosities varied from 0.200 to 0.867, and from 0.186 to 0.739, respectively. All loci were in Hardy–Weinberg equilibrium, and no evidence of linkage disequilibrium was found. Despite its uniqueness, the biota of this area is still understudied and this is the first study describing microsatellite loci for any African species of the Pieridae family, as well as of any butterfly of the study area.

Keywords

Microsatellites Lepidoptera Endemism Afromontane habitats 

Notes

Acknowledgments

Our work was supported by the National Geographic Society (Waitt Grant W163-11), the University of South Bohemia (GAJU 107/2012/P and 168/2013/P), the Institutional Research Support Grant (SVV 260 087/2014) and the Czech Science Foundation (14-36098G).

References

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Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • Jan ZimaJr.
    • 1
    • 2
  • Dan Lestina
    • 2
  • Petr Jansta
    • 3
  • Vojtech Petru
    • 1
  • Robert Tropek
    • 1
    • 4
  1. 1.Institute of Entomology, Biology CentreCzech Academy of ScienceCeske BudejoviceCzech Republic
  2. 2.Faculty of ScienceUniversity of South BohemiaCeske BudejoviceCzech Republic
  3. 3.Department of Zoology, Faculty of ScienceCharles University in PraguePragueCzech Republic
  4. 4.Department of Environmental Science (Biogeography)University of BaselBaselSwitzerland

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