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Conservation Genetics Resources

, Volume 6, Issue 3, pp 661–663 | Cite as

Characterisation of 15 novel microsatellite loci for the grey reef shark (Carcharhinus amblyrhynchos)

  • Paolo Momigliano
  • William D. Robbins
  • Michael Gardner
  • Adam Stow
Microsatellite Letters

Abstract

Grey reef sharks (Carcharhinus amblyrhynchos) are important apex predators on coral reefs, and their numbers have declined dramatically as a result of overfishing. Knowledge of environmental factors that shape gene flow is essential for developing appropriate management strategies, but the lack of suitable genetic markers has hindered research on this species. Here, we characterised 15 polymorphic microsatellite loci for grey reef sharks. None of the loci deviated significantly from Hardy–Weinberg equilibrium and there was no evidence of Linkage Disequilibrium. Several loci cross-amplified in other carcharhinid species, and will be useful in future studies of this family.

Keywords

Grey reef sharks Carcharhinus amblyrhynchos Microsatellites Coral reef 

Notes

Acknowledgments

This study was funded by the Department of Biological Sciences at Macquarie University and the Sea World Research and Rescue Foundation. We would like to thank Siobhan Dennison and Nicolette Armansin for laboratory assistance.

Supplementary material

12686_2014_174_MOESM1_ESM.doc (33 kb)
Supplementary material 1 (DOC 33 kb)
12686_2014_174_MOESM2_ESM.doc (36 kb)
Supplementary material 2 (DOC 36 kb)

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Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • Paolo Momigliano
    • 1
    • 2
  • William D. Robbins
    • 3
    • 4
  • Michael Gardner
    • 5
    • 6
    • 7
  • Adam Stow
    • 1
  1. 1.Conservation Genetics Lab, Department of Biological SciencesMacquarie UniversityNorth RydeAustralia
  2. 2.Sydney Institute of Marine ScienceMosmanAustralia
  3. 3.Wildlife MarinePerthAustralia
  4. 4.School of Marine and Tropical BiologyJames Cook UniversityTownsvilleAustralia
  5. 5.School of Biological SciencesFlinders UniversityAdelaideAustralia
  6. 6.Australian Centre for Evolutionary Biology and BiodiversityUniversity of AdelaideAdelaideAustralia
  7. 7.Evolutionary Biology UnitSouth Australian MuseumNorth Terrace AdelaideAustralia

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