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Conservation Genetics Resources

, Volume 6, Issue 3, pp 507–509 | Cite as

Rapid genetic identification of brown bullhead (Ameiurus nebulosus), black bullhead (Ameiurus melas) and their hybrids

  • R. P. Walter
  • E. S. Gnyra
  • L. I. Söderberg
  • D. D. Heath
Technical Note

Abstract

Hybridization can complicate the effective conservation of cryptic and morphologically similar species. Here we provide a rapid genetic method for the identification of pure-strain brown bullhead (Ameiurus nebulosus) and black bullhead (Ameiurus melas) using nuclear and mitochondrial DNA restriction fragment length polymorphism assays. We confirm earlier reports of introgressive hybridization among A. nebulosus and A. melas in Lake Erie, and reveal the presence of introgressed hybrids in Detroit River. Our method provides a prime example of the utility of internet-based genetic databases for the development of molecular tools in biological and environmental research, and suggests hybridization plays a larger role in the genetic structure of bullheads.

Keywords

Species markers Hybrids SNPs RFLPs 

Notes

Acknowledgments

We thank Mary Burridge and colleagues at the Royal Ontario Museum (ROM) for providing tissue samples for voucher A. melas samples, Vicki Blazer (USGS) for Chesapeake samples, and Jim Grazio for Presque Isle Bay samples.

Supplementary material

12686_2014_161_MOESM1_ESM.doc (38 kb)
Supplementary material 1 (DOC 38 kb)

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Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • R. P. Walter
    • 1
  • E. S. Gnyra
    • 1
  • L. I. Söderberg
    • 2
  • D. D. Heath
    • 1
    • 2
  1. 1.Great Lakes Institute for Environmental ResearchUniversity of WindsorWindsorCanada
  2. 2.Department of Biological SciencesUniversity of WindsorWindsorCanada

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