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Conservation Genetics Resources

, Volume 5, Issue 4, pp 1159–1162 | Cite as

Development of 11 polymorphic microsatellite markers for Xylocarpus granatum (Meliaceae) using next-generation sequencing technology

  • Yuki Tomizawa
  • Yoshimi Shinmura
  • Alison K. S. Wee
  • Koji TakayamaEmail author
  • Takeshi Asakawa
  • Orlex Baylen Yllano
  • Severino Garengo SalmoIII
  • Erwin Riyanto Ardli
  • Nguyen Xuan Tung
  • Norhaslinda Binti Malekal
  • Onrizal
  • Sankararamasubramanian Halasya Meenakshisundaram
  • Sarawood Sungkaew
  • Mohd Nazre Bin Saleh
  • Bayu Adjie
  • Khin Khin Soe
  • Emiko Oguri
  • Noriaki Murakami
  • Yasuyuki Watano
  • Shigeyuki Baba
  • Edward L. Webb
  • Tadashi Kajita
Microsatellite Letters

Abstract

Human impacts have seriously damaged mangroves, and conservation of mangroves will require information on local and regional population genetic structures. Here, we report the development and polymorphism of eleven novel microsatellite markers, developed using next-generation sequencing on 56 samples of widespread mangrove species Xylocarpus granatum (Meliaceae) from nine populations across the Indo-West Pacific region. All loci were found to be polymorphic, with the number of alleles per locus ranging from four to 19. In a population from Sabah (Malaysia), the mean observed and expected heterozygosity per locus was 0.59 and 0.58, respectively. No null allele, significant linkage disequilibrium or deviation from Hardy–Weinberg equilibrium was detected among all loci. The eleven markers developed can be valuable tools to conservation genetics of this species across its distributional range.

Keywords

Conservation Genetic diversity Mangrove Pyrosequencing SSR Indo-West Pacific 

Notes

Acknowledgments

The authors thank Drs. Kyaw Kyaw Khaung and Myint Aung (Myanmar), Mr. Lukes Isechal (Palau), Mr. Cossa de Miranda Filipe (Mozambique), Dr. Jurgenne Primavera (Panay), Dr. Yoichi Tateishi and Mr. Masaharu Amano (University of the Ryukyus), Dr. Monica Suleiman (Sabah), SFD-Sabah Forestry Department (Malaysia), Palau International Coral Reef Center (Palau), Direcção Provincial da Agricultura—Zambézia (Mozambique) and Department of Environment and Natural Resources, Region VI (Panay) for their assistance during field works and material collection. This work was supported by the Singapore Ministry of Education R154-000-440-112 to ELW, by the Fujiwara Natural History Foundation to KT, by the JSPS KAKENHI 22405005 and 25290080 to TK, and JSPS JENESYS Programme 2009 and 2011 to the Graduate School of Science, Chiba University.

Supplementary material

12686_2013_9990_MOESM1_ESM.txt (4 kb)
Supplementary material 1 (TXT 4 kb)

References

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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Yuki Tomizawa
    • 1
  • Yoshimi Shinmura
    • 1
  • Alison K. S. Wee
    • 2
  • Koji Takayama
    • 3
    Email author
  • Takeshi Asakawa
    • 1
  • Orlex Baylen Yllano
    • 4
  • Severino Garengo SalmoIII
    • 5
  • Erwin Riyanto Ardli
    • 6
  • Nguyen Xuan Tung
    • 7
  • Norhaslinda Binti Malekal
    • 8
  • Onrizal
    • 9
  • Sankararamasubramanian Halasya Meenakshisundaram
    • 10
  • Sarawood Sungkaew
    • 11
  • Mohd Nazre Bin Saleh
    • 12
  • Bayu Adjie
    • 13
  • Khin Khin Soe
    • 14
  • Emiko Oguri
    • 15
  • Noriaki Murakami
    • 15
  • Yasuyuki Watano
    • 1
  • Shigeyuki Baba
    • 16
  • Edward L. Webb
    • 2
  • Tadashi Kajita
    • 1
  1. 1.Department of Biology, Graduate School of ScienceChiba UniversityChibaJapan
  2. 2.Department of Biological SciencesNational University of SingaporeSingaporeSingapore
  3. 3.The University MuseumThe University of TokyoTokyoJapan
  4. 4.Biology Department, College of Sciences and TechnologyAdventist University of the PhilippinesSilang CityPhilippines
  5. 5.Department of Environmental Science, School of Science and EngineeringAteneo de Manila UniversityQuezon CityPhilippines
  6. 6.Faculty of BiologyJenderal Soedirman UniversityPurwokertoIndonesia
  7. 7.Mangrove Ecosystem Research CentreHanoi National University of EducationHanoiVietnam
  8. 8.Institute for Tropical Biology and ConservationUniversiti Malaysia SabahKota KinabaluMalaysia
  9. 9.Forestry Sciences DepartmentUniversitas Sumatera UtaraMedanIndonesia
  10. 10.Biotechnology ProgrammeSwaminathan Research FoundationChennaiIndia
  11. 11.Faculty of ForestryKasesart UniversityBangkokThailand
  12. 12.Department of Forest Management, Faculty of ForestryUniversiti Putra MalaysiaSelangorMalaysia
  13. 13.Bali Botanical GardenBaliIndonesia
  14. 14.Department of BotanyUniversity of YangonYangonMyanmar
  15. 15.Department of Biological SciencesTokyo Metropolitan UniversityTokyoJapan
  16. 16.Tropical Biosphere Research CenterUniversity of the RyukyusIriomote, OkinawaJapan

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