Development of 28 polymorphic microsatellite markers for the endemic Azorean spider Sancus acoreensis (Araneae, Tetragnathidae)
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We isolated and characterized a total of 28 microsatellite loci from Sancus acoreensis. Loci were screened in 26 individuals originating from seven (Flores, Faial, Pico, São Jorge, Terceira, São Miguel, and Santa Maria) out of the nine islands of the Azores. The number of alleles per locus ranged from 2 to 14, observed heterozygosity ranged from 0.040 to 0.708, and the probability of identity values ranged from 0.02 to 0.97. Sancus acoreensis is a Laurel forest specialist species, endemic to the Azores, and is facing a great extinction risk due to the severe fragmentation of its habitat. The newly developed microsatellite loci will aid in detecting signs of population bottlenecks and pinpoint the island populations that are facing the greatest risk of extinction.
Sancus acoreensis(Araneae, Tetragnathidae) is an endemic Azorean species that inhabits the Laurel forest remnants within the Azores. It is facing a high risk of extinction due to the fragmentation and degradation of its...
KeywordsExtinction Risk Laurel Forest Illumina TruSeq Genetic Diversity Level Adaptor Index
We would like to express our gratitude to Carla Rego, Luís Crespo, Pedro Cardoso and Isabel Amorim for providing material and ideas during the course of this study. Funded was provided by Fundação para a Ciência e Tecnologia (project: PTDC/BIA-BEC/100182/2008). Manuscript preparation was partially supported by the US Department of Energy (award number DE-FC09-07SR22506) to the University of Georgia Research Foundation.
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