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Conservation Genetics Resources

, Volume 5, Issue 4, pp 1133–1134 | Cite as

Development of 28 polymorphic microsatellite markers for the endemic Azorean spider Sancus acoreensis (Araneae, Tetragnathidae)

  • Aristeidis ParmakelisEmail author
  • Katerina Balanika
  • Sofia Terzopoulou
  • Francois Rigal
  • Rochelle R. Beasley
  • Kenneth L. Jones
  • Stacey L. Lance
  • Robert J. Whittaker
  • Kostas A. Triantis
  • Paulo A.V. Borges
Microsatellite Letters

We isolated and characterized a total of 28 microsatellite loci from Sancus acoreensis. Loci were screened in 26 individuals originating from seven (Flores, Faial, Pico, São Jorge, Terceira, São Miguel, and Santa Maria) out of the nine islands of the Azores. The number of alleles per locus ranged from 2 to 14, observed heterozygosity ranged from 0.040 to 0.708, and the probability of identity values ranged from 0.02 to 0.97. Sancus acoreensis is a Laurel forest specialist species, endemic to the Azores, and is facing a great extinction risk due to the severe fragmentation of its habitat. The newly developed microsatellite loci will aid in detecting signs of population bottlenecks and pinpoint the island populations that are facing the greatest risk of extinction.

Sancus acoreensis(Araneae, Tetragnathidae) is an endemic Azorean species that inhabits the Laurel forest remnants within the Azores. It is facing a high risk of extinction due to the fragmentation and degradation of its...

Keywords

Extinction Risk Laurel Forest Illumina TruSeq Genetic Diversity Level Adaptor Index 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

We would like to express our gratitude to Carla Rego, Luís Crespo, Pedro Cardoso and Isabel Amorim for providing material and ideas during the course of this study. Funded was provided by Fundação para a Ciência e Tecnologia (project: PTDC/BIA-BEC/100182/2008). Manuscript preparation was partially supported by the US Department of Energy (award number DE-FC09-07SR22506) to the University of Georgia Research Foundation.

Supplementary material

12686_2013_9976_MOESM1_ESM.doc (44 kb)
Supplementary material 1 (DOC 43 kb)

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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Aristeidis Parmakelis
    • 1
    • 2
    Email author
  • Katerina Balanika
    • 1
  • Sofia Terzopoulou
    • 1
    • 2
  • Francois Rigal
    • 2
  • Rochelle R. Beasley
    • 3
  • Kenneth L. Jones
    • 4
  • Stacey L. Lance
    • 3
  • Robert J. Whittaker
    • 5
    • 6
  • Kostas A. Triantis
    • 1
    • 2
    • 5
  • Paulo A.V. Borges
    • 2
  1. 1.Department of Ecology and Taxonomy, Faculty of BiologyUniversity of AthensAthensGreece
  2. 2.Departamento de Ciências. AgráriasAzorean Biodiversity Group (CITA-A) and Platform for Ecological and Environmental Research (PEER)Universidade dos AçoresAngra do HeroismoPortugal
  3. 3.Savannah River Ecology LaboratoryUniversity of GeorgiaAikenUSA
  4. 4.Department of Biochemistry and Molecular GeneticsUniversity of Colorado School of MedicineAuroraUSA
  5. 5.Conservation Biogeography and Macroecology ProgrammeOxford University Centre for the EnvironmentOxfordUK
  6. 6.Center for Macroecology Evolution and Climate, Department of BiologyUniversity of CopenhagenCopenhagenDenmark

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