Conservation Genetics Resources

, Volume 5, Issue 4, pp 1121–1123 | Cite as

Characterization of STR/microsatellite primers for the Gila monster, Heloderma suspectum screened from paired-end Illumina shotgun sequencing

  • Michael R. Hess
  • Taylor Edwards
  • David A. Edmunds
  • Kevin E. Bonine
Microsatellite Letters

Abstract

We used Illumina paired-end shotgun sequencing to characterize microsatellite loci in Heloderma suspectum. We identified over 124,000 potentially amplifiable loci and describe PCR primers for 18 variable tri- and tetra-nucleotide STRs. The observed number of alleles per locus ranged from 5 to 16 and heterozygosity varied from 0.64 to 0.92. In addition 13 of these loci cross-amplified in the beaded lizard, Heloderma horridum. This method of microsatellite identification proved extremely efficient and cost effective. This novel marker set can be used by researchers to better understand these elusive species.

Keywords

Heloderma Microsatellite Illumina HiSeq PCR primers STR 

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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Michael R. Hess
    • 1
  • Taylor Edwards
    • 2
  • David A. Edmunds
    • 3
  • Kevin E. Bonine
    • 3
  1. 1.Department of Molecular and Cellular BiologyUniversity of ArizonaTucsonUSA
  2. 2.Arizona Research LaboratoriesUniversity of Arizona Genetics CoreTucsonUSA
  3. 3.Department of Ecology and Evolutionary BiologyUniversity of ArizonaTucsonUSA

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