Advertisement

Conservation Genetics Resources

, Volume 5, Issue 4, pp 1117–1120 | Cite as

Development and characterisation of microsatellite loci for Murina gracilis (Vespertilionidae) and cross-species amplification in two other congeneric species

  • Hao-Chih Kuo
  • Shiang-Fan Chen
  • Stephen J. Rossiter
Microsatellite Letters

Abstract

Tube-nosed bats (Murina) are restricted to the forests of Asia, Papua New Guinea and northeast Australia, where they are considered highly vulnerable to habitat loss and fragmentation. Several members of the genus have been recently discovered and remain poorly known, including the putative sister species Murina gracilis and M. recondita, both of which are endemic to Taiwan. We developed novel microsatellite markers for M. gracilis and screened these in both taxa and a third Taiwanese endemic M. puta. The final number of useable markers that did not deviate from Hardy–Weinberg or linkage equilibrium was 18 for M. gracilis and M. recondita, including one that was X-linked, and nine for M. puta. We are using these markers to characterise population genetic structure and gene flow within all three taxa to inform conservation management.

Keywords

Tube-nosed bats Taiwan Conservation genetics 

Notes

Acknowledgments

We thank Cheng-Han Chou, Chia-Hong Chen, Ying-Ping Fang and Ling–Ling Lee for donating tissue samples. This study was supported by the University of London Central Research Fund (UK) and both Taipei Zoo and National Science Council (NSC 99-2621-B-126-001) from Taiwan.

Supplementary material

12686_2013_9970_MOESM1_ESM.pdf (36 kb)
Supplementary material 1 (PDF 36 kb)

References

  1. Brownstein MJ, Carpten JD, Smith JR (1996) Modulation of non-templated nucleotide addition by Taq DNA polymerase: primer modifications that facilitate genotyping. Biotechniques 20:1004–1010PubMedGoogle Scholar
  2. Francis CM, Eger JL (2012) A review of tube-nosed bats (Murina) from Laos with a description of two new species. Acta Chiropterol 14:15–38CrossRefGoogle Scholar
  3. Hsu RC-C, Tamis WLM, Raes N, de Snoo GR, Wolf JHD, Oostermeijer G, Lin S-H (2012) Simulating climate change impacts on forests and associated vascular epiphytes in a subtropical island of East Asia. Divers Distrib 18:334–347CrossRefGoogle Scholar
  4. Kingston T, Francis CM, Akbar Z, Kunz TH (2003) Species richness in an insectivorous bat assemblage from Malaysia. J Trop Ecol 19:67–79CrossRefGoogle Scholar
  5. Kuo H-C, Fang Y-P, Csorba G, Lee L-L (2009) Three new species of Murina (Chiroptera: Vespertilionidae) from Taiwan. J Mammal 90:980–991CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Hao-Chih Kuo
    • 1
  • Shiang-Fan Chen
    • 2
  • Stephen J. Rossiter
    • 1
  1. 1.School of Biological and Chemical SciencesQueen Mary University of LondonLondonUK
  2. 2.Center for General EducationNational Taipei UniversityNew Taipei CityTaiwan

Personalised recommendations