Development and cross-species transferability of 23 microsatellite markers from the vulnerable Chinese Egret (Egretta eulophotes) using 454 sequencing
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The Chinese Egret (Egretta eulophotes) is a vulnerable waterbird breeding on offshore islands. Here, 23 new microsatellite loci were isolated using 454 pyrosequencing, and were tested in 32 individuals from a single population in Fujian province of China. The number of alleles per locus ranged from 2 to 9. Observed heterozygosity and expected heterozygosity ranged from 0.094 to 0.844 and 0.091 to 0.840, respectively. All loci showed Hardy–Weinberg equilibrium with the exception of three loci (Ee10, Ee17 and Ee23), possibly because of null alleles. The new loci also were confirmed a high cross-amplification success rate in the other seven Ardeid species. These new microsatellite data will provide a major resource for future conservation genetics studies on this vulnerable egret and the other Ardeids.
KeywordsMicrosatellite 454 Pyrosequencing Cross-amplification Bird Egret Heron
We thank Zeng Wang, Peng Cai, Wei Lei, Jing Lin and Changfu Mao who helped collect some samples for this study. This research was supported by the National Natural Science Foundation of China (Grant Nos. 30970380, 31000963 and 31272333) and by the Fujian or Henan Natural Science Foundation of China (2010Y2007, 2011J01247, 2012J01149 and 102102110180).
- BirdLife International (2013) Species factsheet: Egretta eulophotes. Downloaded from http://www.birdlife.org on 1 May 2013