Characterization of 9 polymorphic microsatellite loci in Lamellibrachia sp. 2, a tubeworm found at deep-sea hydrothermal vents and cold seeps
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Lamellibrachia sp. 2 is a deep-sea vestimentiferan tubeworm found at hydrothermal vents on the Mid-Cayman Spreading Center and at hydrocarbon seeps in the Gulf of Mexico and off the coast of Barbados. Nine selectively neutral and unlinked polymorphic microsatellite loci were developed for this species. Eight of these loci conformed to Hardy–Weinberg expectations. Average observed heterozygosity ranged from 0.14 to 0.92. Microsatellites developed for Lamellibrachia sp. 2 are being deployed to study connectivity and gene flow among populations of this species.
KeywordsMid-Cayman Spreading Center Von Damm vent field Barbados seeps Gulf of Mexico seeps Vestimentifera Gene flow Connectivity
This research was supported by NSF award OCE-1031050 and NASA ASTEP subcontract through WHOI (NNX09AB75G) to CLVD. We are thankful to the Chief Scientist, C. German, the crew of the R/V Atlantis (AT18-16) and the crew of the ROV Jason. We also acknowledge Mengyou Wu for help with DNA extractions. SP, ADT, CLVD conceived the study; SP, CLVD collected samples, SP and AJ undertook molecular benchwork; AJ, SP, ADT analyzed the data; AJ, SP, ADT, and CLVD drafted the manuscript.
- Jacobson A, Plouviez S, Thaler AD, Van Dover CL (2013) Characterization of 13 polymorphic microsatellite loci in Rimicaris hybisae, a shrimp from deep-sea hydrothermal vents. Conservation Genet Resour. 5(2):449–451. doi: 10.1007/s12686-012-9825-0
- Plouviez S, Shank TM, Faure B, Daguin-Thiébaut C, Viard F, Lallier FH, Jollivet D (2009) Comparative phylogeography among hydrothermal vent species along the East Pacific Rise reveals vicariant processes and population expansion in the South. Mol Ecol. doi: 10.1111/j.1365-294X.2009.04325.x PubMedGoogle Scholar
- Rozen S, Skaletsky HJ (2000) Primer3 on the WWW for general users and for biologist programmers. In: Krawetz S, Misener S (eds) Bioinformatics methods and protocols: methods in molecular biology. Humana Press, TotowaGoogle Scholar