Conservation Genetics Resources

, Volume 5, Issue 4, pp 907–909

Development and characterization of ten polymorphic microsatellite loci for the Great Capricorn beetle (Cerambyx cerdo) (Coleoptera: Cerambycidae)

Technical Note

Abstract

Ten polymorphic microsatellite loci, developed using next-generation sequencing technology, are described for the Great Capricorn beetle, Cerambyx cerdo; an endangered and internationally protected European longicorn. Based on 30 individuals from South-East Czech Republic, the number of alleles per locus ranged from 4 to 8, and the observed and expected heterozygosity was 0.36–0.79 (mean = 0.57) and 0.5–0.77 (mean = 0.63), respectively. In one locus tests indicated a deviation from the Hardy–Weinberg equilibrium, as well as the presence of null alleles. Future studies using markers described here may provide additional information for the efficient protection of this threatened species.

Keywords

Xylophagous insect Roche 454 SSR markers Population genetics 

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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  1. 1.Faculty of SciencesUniversity of South BohemiaCeske BudejoviceCzech Republic
  2. 2.Institute of Entomology, Biology CentreCzech Academy of SciencesCeske BudejoviceCzech Republic

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