Development and characterization of ten polymorphic microsatellite loci for the Great Capricorn beetle (Cerambyx cerdo) (Coleoptera: Cerambycidae)
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Ten polymorphic microsatellite loci, developed using next-generation sequencing technology, are described for the Great Capricorn beetle, Cerambyx cerdo; an endangered and internationally protected European longicorn. Based on 30 individuals from South-East Czech Republic, the number of alleles per locus ranged from 4 to 8, and the observed and expected heterozygosity was 0.36–0.79 (mean = 0.57) and 0.5–0.77 (mean = 0.63), respectively. In one locus tests indicated a deviation from the Hardy–Weinberg equilibrium, as well as the presence of null alleles. Future studies using markers described here may provide additional information for the efficient protection of this threatened species.
KeywordsXylophagous insect Roche 454 SSR markers Population genetics
We would like to thank Matthew Sweney for language correction, Jaroslav Vohánka and Lucie Vondráčková (both Roche Ltd., Prague) for GS Junior Sequencer demo runs and assistance, and Michal Plátek for support. This study and its authors were supported by the Technology Agency of the Czech Republic (TA02021501), the Grant Agency of the University of South Bohemia (168/2013/P), the project Biodiversity of Forest Ecosystems CZ.1.07/2.3.00/20.0064, and the Higher Education Development Fund from the Ministry of Education, Youth and Sports of the Czech Republic (1904/2012).
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