Conservation Genetics Resources

, Volume 5, Issue 4, pp 903–905 | Cite as

Characterization of nine polymorphic microsatellite loci for a threatened saproxylic beetle Rosalia alpina (Coleoptera: Cerambycidae)

Technical Note

Abstract

Nine polymorphic microsatellite loci were developed and characterized for the Rosalia longicorn, an endangered icon of European saproxylic biodiversity. The detected number of alleles per locus ranged from 2 to 4, and the observed and expected heterozygosities varied from 0.044 to 0.622, and from 0.086 to 0.613, respectively. All loci were in Hardy–Weinberg equilibrium as well, as no evidence of linkage disequilibrium was found. Despite the low level of polymorphism, all loci described in this study will provide a useful tool in future genetic studies of the Rosalia alpina species.

Keywords

SSR markers Population genetics Dead wood Xylophagous 

References

  1. Bense U (2002) Schutzmaßnahmen für den Alpenbock (Rosalia alpina) im Bereich der Schwäbischen Alb. DgaaE Nachrichten 16:57–58Google Scholar
  2. Bosso L, Rebelo H, Garonna AP, Russo D (2013) Modelling geographic distribution and detecting conservation gaps in Italy for the threatened beetle Rosalia alpina. J Nat Conserv 21:72–80CrossRefGoogle Scholar
  3. Ciach M, Michalcewicz J, Fluda M (2007) The first report on development of Rosalia alpina (Linnaeus, 1758) (Coleoptera: Cerambycidae) in wood of Ulmus L. in Poland. Pol J Entomol 76:101–105Google Scholar
  4. Cizek L, Schlaghamerský J, Bořucký J, Hauck D, Helešic J (2009) Range expansion of an endangered beetle: alpine longhorn Rosalia alpina (Coleoptera: Cerambycidae) spreads to the lowlands of Central Europe. Entomol Fennica 20:200–206Google Scholar
  5. Dakin EE, Avise JC (2004) Microsatellite null alleles in parentage analysis. Hered 93:504–509CrossRefGoogle Scholar
  6. Drag L, Hauck D, Pokluda P, Zimmermann K, Cizek L (2011) Demography and dispersal ability of a threatened Saproxylic Beetle: a mark-recapture study of the Rosalia Longicorn (Rosalia alpina). PLoS ONE 6(6):e21345CrossRefPubMedGoogle Scholar
  7. Duelli P, Wermelinger B (2005) Der Alpenbock (Rosalia alpina). Ein seltener Bockkäfer als Flaggschiff–Art. Merkblatt für die Praxis 39:1–8Google Scholar
  8. Frankham R (2008) Genetic adaptation to captivity in species conservation programs. Mol Ecol 17:325–333CrossRefPubMedGoogle Scholar
  9. IUCN (2012) IUCN red list of threatened species. Version 2012.2. www.iucnredlist.org. Accessed 13 March 2013
  10. Lachat T, Ecker K, Duelli P, Wermelinger B (2013) Population trends of Rosalia alpina (L.) in Switzerland: a lasting turnaround? J Insect Conserv. doi:10.1007/s10841-013-9549-9
  11. Lindhe A, Jeppsson T, Ehnström B (2010) Longhorn beetles in Sweden—changes in distribution and abundance over the last two hundred years. (Långhorningar i Sverige—förändringar av utbredning och frekvens under de senaste tvåhundra åren.) Entomologisk Tidskrift, Uppsala, SwedenGoogle Scholar
  12. Malausa T, Gilles A, Meglécz E et al (2011) High-throughput microsatellite isolation through 454 GS-FLX Titanium pyrosequencing of enriched DNA libraries. Mol Ecol Res 11:638–644CrossRefGoogle Scholar
  13. Peakall R, Smouse PE (2006) GENALEX 6: genetic analysis in Excel. Population genetic software for teaching and research. Mol Ecol Notes 6:288–295CrossRefGoogle Scholar
  14. Rousset F (2008) Genepop’007: a complete re-implementation of the genepop software for Windows and Linux. Mol Ecol Resour 8:103–106CrossRefPubMedGoogle Scholar
  15. Russo D, Cistrone L, Garonna AP (2011) Habitat selection by the highly endangered long-horned beetle Rosalia alpina in Southern Europe: a multiple spatial scale assessment. J Insect Conserv 15:685–693CrossRefGoogle Scholar
  16. Sama G (2002) Atlas of Cerambycidae of Europe and the Mediterranean Area. Vol. I. Northern, Western, Central and Eastern Europe, British Isles and Continental Europe from France (excl. Corsica) to Scandinavia and the Urals. Kabourek, ZlínGoogle Scholar
  17. Sama G, Rapuzzi P, Özdikmen H (2012) Preliminary report of the entomological surveys (2010, 2011) of G. Sama and P. Rapuzzi to Turkey (Coleoptera: Cerambycidae). Munis Entomol Zool 7:22–45Google Scholar
  18. Starzyk JR (2004) Rosalia alpina (Linnaeus, 1758), Nadobnica alpejska. In: Głowacinski Z, Nowacki J (ed) Polska czerwona ksiega zwierzat. Bezkregowce. (Polish red data book of animals. Invertebrates.) Kraków: IOP PAN, AR Poznań. pp 148–149Google Scholar
  19. Van Oosterhout C, Hutchison WF, Wills DPM, Shipley P (2004) Micro-checker: software for identifying and correcting genotyping errors in microsatellite data. Mol Ecol Notes 4:535–538CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  1. 1.Faculty of SciencesUniversity of South BohemiaCeske BudejoviceCzech Republic
  2. 2.Institute of Entomology, Biology CentreCzech Academy of SciencesCeske BudejoviceCzech Republic

Personalised recommendations