Conservation Genetics Resources

, Volume 5, Issue 4, pp 903–905 | Cite as

Characterization of nine polymorphic microsatellite loci for a threatened saproxylic beetle Rosalia alpina (Coleoptera: Cerambycidae)

  • L. Drag
  • J. ZimaJr.
  • L. Cizek
Technical Note


Nine polymorphic microsatellite loci were developed and characterized for the Rosalia longicorn, an endangered icon of European saproxylic biodiversity. The detected number of alleles per locus ranged from 2 to 4, and the observed and expected heterozygosities varied from 0.044 to 0.622, and from 0.086 to 0.613, respectively. All loci were in Hardy–Weinberg equilibrium as well, as no evidence of linkage disequilibrium was found. Despite the low level of polymorphism, all loci described in this study will provide a useful tool in future genetic studies of the Rosalia alpina species.


SSR markers Population genetics Dead wood Xylophagous 



We would like to thank Matthew Sweney for language corrections and Beata Baranová for support. This study and its authors were supported by the Technology Agency of the Czech Republic (TA02021501), the Grant Agency of the University of South Bohemia (168/2013/P), and the project Biodiversity of forest ecosystems CZ.1.07/2.3.00/20.0064.


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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  1. 1.Faculty of SciencesUniversity of South BohemiaCeske BudejoviceCzech Republic
  2. 2.Institute of Entomology, Biology CentreCzech Academy of SciencesCeske BudejoviceCzech Republic

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