Conservation Genetics Resources

, Volume 5, Issue 3, pp 787–790 | Cite as

Novel microsatellite DNA markers for Indian Gharial (Gavialis gangeticus)

  • K. Naga Jogayya
  • P. R. Meganathan
  • Bhawna Dubey
  • I. Haque
Technical note
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Abstract

The present status of existing crocodile species has increased the necessity to develop novel utilities for conservation. We have developed 18 microsatellite loci from the Indian Gharial (Gavialis gangeticus) intended for genomic explanation and applied them to study genetic variation. Polymorphism of each locus was assessed in 32 individuals for G. gangeticus India. The number of alleles per locus varied from 2 to 8 (mean 5.5) for Indian population. Observed and expected heterozygosity ranged from 0.73 to 1.00 and 0.50 to 0.81, respectively and the average polymorphic information content is 0.565. These selected markers are helpful for assessing population structure, intraspecific difference, and conservation and management of G. gangeticus.

Keywords

Indian Gharial Microsatellite development Gavialis gangeticus 
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Notes

Acknowledgments

The authors are thankful to Madras Crocodile Bank Trust (MCBT), Centre for Herpetology, Mamallapuram, Tamilnadu, India, National Chambal Sanctuary Project, Agra, Uttar Pradesh and Nehru Zoological Park, Hyderabad, Andhra Pradesh, India for providing authenticated crocodilian samples for this study. This study was funded by Directorate of Forensic Science Services, Ministry of Home Affairs, Government of India, New Delhi.

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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • K. Naga Jogayya
    • 1
  • P. R. Meganathan
    • 1
    • 2
  • Bhawna Dubey
    • 1
    • 3
  • I. Haque
    • 1
    • 4
  1. 1.Central Forensic Science LaboratoryBiology DivisionKolkataIndia
  2. 2.Institute for Genomics, Biocomputing and BiotechnologyMississippi State UniversityStarkvilleUSA
  3. 3.StarkvilleUSA
  4. 4.Central Forensic InstituteGuwahatiIndia

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