Conservation Genetics Resources

, Volume 5, Issue 3, pp 647–649 | Cite as

Characterization of 10 polymorphic microsatellite loci in Munidopsis lauensis, a squat-lobster from the southwestern Pacific

  • Emily A. Boyle
  • Andrew David ThalerEmail author
  • Alixandra Jacobson
  • Sophie Plouviez
  • Cindy Lee Van Dover
Technical Note


Munidopsis lauensis is a deep-sea squat lobster commonly associated with hydrothermal vents at southwest Pacific back-arc spreading centers. Eight selectively neutral and unlinked polymorphic microsatellite loci were developed for this species and two additional loci from a related species were successfully cross-amplified. Eight of the ten total loci conformed to Hardy–Weinberg expectations. Average observed heterozygosity ranged from 0.23 to 0.64 (mean H O  = 0.50, SD = 0.15). Seven of ten loci cross-amplified in Munidopsis antonii, a closely related species. Microsatellites developed for M. lauensis are being deployed to study connectivity among populations of this species that occur at hydrothermal vents in Manus and Lau Basins.


Munidopsis antonii Hydrothermal vent Back-arc basin Manus Basin 



This research was supported by a research contract from Nautilus Minerals Niugini Limited to CLVD. The authors thank Dr. Samantha Smith of Nautilus Minerals, the captain and crew of the M/V Nor Sky, the Canyon Offshore ROV team, Rebecca Jones and Pen-Yuan Hsing for assistance with field sampling, and Freddie Alei and Bernard Ball for assistance with bench work. Munidopsis lauensis samples from Manus Basin are the property of Papua New Guinea, are held in trust by Nautilus Minerals, and on loan to Duke University for baseline studies for the Solwara 1 Project. ADT, SP, CLVD conceived the study; ADT collected samples; EAB, AJ undertook molecular bench work; EAB, ADT, AJ, SP analyzed the data; EAB, ADT, AJ, SP, CLVD drafted the manuscript. All authors have read and approved the final manuscript.


  1. Antao T, Lopes RJ, Beja-Pereira A, Luikart G (2008) LOSITAN: a workbench to detect molecular adaptation based on a Fst-outlier method. BMC Bioinformatics 9:323PubMedCrossRefGoogle Scholar
  2. Baba K, de Saint Laurent M (1992) Chirostylid and galatheid crustaceans (Decapoda: Anomura) from active thermal vent areas in the SW Pacific. Sci Mar 56:321–332Google Scholar
  3. Bailie DA, Hynes R, Prodöhl PA (2011) Genetic parentage in the squat lobsters Munida rugosa and M. sarsi (Crustacea, Anomura, Galatheidae). Mar Ecol Prog Ser 421:173–182CrossRefGoogle Scholar
  4. Beaumont MA, Nicholas RA (1996) Evaluating loci for use in the genetic analysis of population structure. Proc R Soc Lond B 263:1619–1626CrossRefGoogle Scholar
  5. Cabezas P, Bloor P, Acevedo I, Toledo C, Calvo M, Macpherson E, Machordom A (2009) Development and characterization of microsatellite markers for the endangered anchialine squat lobster Munidopsis polymorpha. Conserv Genet 10:673–676CrossRefGoogle Scholar
  6. Faircloth BC (2008) MSATCOMMANDER: detection of microsatellite repeat arrays and automated, locus-specific primer design. Mol Ecol Resour 8:92–94PubMedCrossRefGoogle Scholar
  7. Kalinowski ST (2006) HW-QuickCheck: an easy-to-use computer program for checking genotypes for agreement with Hardy–Weinberg expectations. Mol Ecol Notes 6:974–979CrossRefGoogle Scholar
  8. Rice WR (1989) Analyzing tables of statistical tests. Evolution 43(1):223–225CrossRefGoogle Scholar
  9. Rozen S, Skaletsky HJ (2000) Primer3 on the WWW for general users and for biologist programmers. In: Krawetz S, Misener S (eds) Bioinformatics methods and protocols: methods in molecular biology. Humana Press, TotowaGoogle Scholar
  10. Schuelke M (2000) An economic method for the fluorescent labeling of PCR fragments. Nat Biotechnol 18:233–234PubMedCrossRefGoogle Scholar
  11. Schultz T, Hsing P, Eng A, Zelnio K (2010) Characterization of 18 polymorphic microsatellite loci from Bathymodiolus manusensis (Bivalvia, Mytilidae) from deep-sea hydrothermal vents. Conserv Genet Resour 3:25–27CrossRefGoogle Scholar
  12. Thaler AD, Zelnio K, Jones R, Carlsson J, Van Dover CL, Schultz TF (2010) Characterization of 12 polymorphic microsatellite loci in Ifremeria nautilei, a chemoautotrophic gastropod from deep-sea hydrothermal vents. Conserv Genet Resour 2:101–103CrossRefGoogle Scholar
  13. Thaler AD, Zelnio K, Saleu W, Schultz TF, Carlsson J, Cunningham C, Vrijenhoek RC, Van Dover CL (2011) The spatial scale of genetic subdivision in populations of Ifremeria nautilei, a hydrothermal-vent gastropod from the southwest Pacific. BMC Evol Biol 11(1):372PubMedCrossRefGoogle Scholar
  14. van Oosterhout C, Hutchinson W, Willds D, Shipley P (2004) MICRO-CHECKER: software for identifying and correcting genotyping errors in microsatellite data. Mol Ecol Notes 4:535–538CrossRefGoogle Scholar
  15. Vrijenhoek RC (1997) Gene flow and genetic diversity in naturally fragmented metapopulations of deep-sea hydrothermal vent animals. J Hered 88(4):285–293Google Scholar
  16. Vrijenhoek RC (2010) Genetic diversity and connectivity of deep sea hydrothermal vent metapopulations. Mol Ecol 19(20):4391–4411Google Scholar
  17. Zelnio KA, Thaler AD, Jones RE, Saleu W, Schultz TF, Van Dover CL, Carlsson J (2010) Characterization of nine polymorphic microsatellite loci in Chorocaris sp. (Crustacea, Caridea, Alvinocarididae) from deep-sea hydrothermal vents. Conserv Genet Resour 2(1):223–226CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Emily A. Boyle
    • 1
  • Andrew David Thaler
    • 1
    Email author
  • Alixandra Jacobson
    • 1
  • Sophie Plouviez
    • 1
  • Cindy Lee Van Dover
    • 1
  1. 1.Marine Laboratory, Nicholas School of the EnvironmentDuke UniversityBeaufortUSA

Personalised recommendations