Characterisation of polymorphic microsatellite loci for the bryozoan Fredericella sultana, the primary host of the causative agent of salmonid proliferative kidney disease
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The freshwater bryozoan Fredericella sultana is the most common primary host for the myxozoan parasite Tetracapsuloides bryosalmonae, causing proliferative kidney disease (PKD) in salmonid fish. An Illumina next-generation sequencing library containing genomic DNA from both T. bryosalmonae and F. sultana yielded 10,653 microsatellite motifs. Twenty-four loci were tested in both species and 12 novel polymorphic tri- and tetranucleotide microsatellite markers were developed for F. sultana. These markers provide a valuable resource for population genetic studies of F. sultana, whilst the apparent lack of microsatellites in the PKD agent suggests a low frequency of tri- and tetranucleotide repeats in the T. bryosalmonae genome.
KeywordsPhylactolaemata Myxozoa Tetracapsuloides bryosalmonae PKD Population genetics
We thank Meri Lindqvist (University of Turku, Finland) for her help using msatcommander software. This work was supported by Academy of Finland (AV), Estonian Science Foundation (Grant Number 8215 to AV), Erasmus Mundus research mobility (project 2011467 to DF) and research funds from the Natural History Museum, London (HH, BO).