Conservation Genetics Resources

, Volume 6, Issue 2, pp 481–482 | Cite as

Characterisation of polymorphic microsatellite loci for the bryozoan Fredericella sultana, the primary host of the causative agent of salmonid proliferative kidney disease

  • Dmitry Filippenko
  • Hanna Hartikainen
  • Beth Okamura
  • Anti VasemägiEmail author
Microsatellite Letters


The freshwater bryozoan Fredericella sultana is the most common primary host for the myxozoan parasite Tetracapsuloides bryosalmonae, causing proliferative kidney disease (PKD) in salmonid fish. An Illumina next-generation sequencing library containing genomic DNA from both T. bryosalmonae and F. sultana yielded 10,653 microsatellite motifs. Twenty-four loci were tested in both species and 12 novel polymorphic tri- and tetranucleotide microsatellite markers were developed for F. sultana. These markers provide a valuable resource for population genetic studies of F. sultana, whilst the apparent lack of microsatellites in the PKD agent suggests a low frequency of tri- and tetranucleotide repeats in the T. bryosalmonae genome.


Phylactolaemata Myxozoa Tetracapsuloides bryosalmonae PKD Population genetics 



We thank Meri Lindqvist (University of Turku, Finland) for her help using msatcommander software. This work was supported by Academy of Finland (AV), Estonian Science Foundation (Grant Number 8215 to AV), Erasmus Mundus research mobility (project 2011467 to DF) and research funds from the Natural History Museum, London (HH, BO).

Supplementary material

12686_2013_133_MOESM1_ESM.xls (666 kb)
Supplementary material 1 (XLS 665 kb)
12686_2013_133_MOESM2_ESM.xls (46 kb)
Supplementary material 2 (XLS 46 kb)


  1. Hartikainen H, Jokela J (2012) Characterisation of polymorphic microsatellite loci in the freshwater bryozoan Fredericella sultana. Conserv Genet Resour 4:457–477. doi: 10.1007/s12686-011-9578-1 CrossRefGoogle Scholar
  2. Okamura B, Wood TS (2002) Bryozoans as hosts for Tetracapsula bryosalmonae, the PKX organism. J Fish Dis 25:1–7CrossRefGoogle Scholar
  3. Okamura B, Hartikainen H, Schmidt-Posthaus H, Wahl T (2010) Life cycle complexity, environmental change and the emerging status of salmonid proliferative kidney disease. Fresh Biol 56:735–753. doi: 10.1111/j.1365-2427.2010.02465.x CrossRefGoogle Scholar
  4. Schmieder R, Edwards R (2011) Quality control and preprocessing of metagenomic datasets. Bioinformatics 27:863–864. doi: 10.1093/bioinformatics/btr026 PubMedCentralPubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • Dmitry Filippenko
    • 1
    • 2
  • Hanna Hartikainen
    • 3
  • Beth Okamura
    • 3
  • Anti Vasemägi
    • 1
    • 4
    Email author
  1. 1.Division of Genetics and Physiology, Department of BiologyUniversity of TurkuTurkuFinland
  2. 2.Department of Ichthyopathology and HydrobiologyKaliningrad State Technical UniversityKaliningradRussian Federation
  3. 3.Department of Life SciencesNatural History MuseumLondonUK
  4. 4.Department of AquacultureEstonian University of Life SciencesTartuEstonia

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