Conservation Genetics Resources

, Volume 6, Issue 1, pp 175–177 | Cite as

Development and characterization of microsatellite loci for two species of Beringian birds, rock sandpiper (Calidris ptilocnemis) and Pacific wren (Troglodytes pacificus)

  • Christin L. Pruett
  • Cesili Whelan
  • Angela Ricono
  • Stacey L. Lance
  • Travis Glenn
  • Brant Faircloth
  • Kevin Winker
Microsatellite Letters

Abstract

Identification and assessment of small, endemic populations are priorities for conservation. We isolated and characterized 8 microsatellite loci from rock sandpiper (Calidris ptilocnemis) and 5 microsatellite loci from Pacific wren (Troglodytes pacificus), species with endemic populations of named subspecies that are of conservation concern. Eighteen to 20 individuals of each species from several locations in Alaska were screened for polymorphism. Loci for each species showed high polymorphism, with rock sandpiper ranging from 5 to 14 alleles per locus and 0.73–0.88 expected heterozygosity and Pacific wren ranging from 5 to 14 alleles per locus and 0.55–0.91 expected heterozygosity. Loci developed for rock sandpipers were also polymorphic in closely related taxa. These loci are the first developed for either species and will be used to identify and conserve endemic populations in the Bering Sea region.

Keywords

Troglodytes Calidris PCR primers Microsatellite Aleutian Islands Pribilof Islands 

Supplementary material

12686_2013_40_MOESM1_ESM.doc (50 kb)
Supplementary material 1 (DOC 50 kb)

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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Christin L. Pruett
    • 1
  • Cesili Whelan
    • 1
  • Angela Ricono
    • 1
  • Stacey L. Lance
    • 2
  • Travis Glenn
    • 3
  • Brant Faircloth
    • 4
  • Kevin Winker
    • 5
  1. 1.Department of Biological SciencesFlorida Institute of TechnologyMelbourneUSA
  2. 2.Savannah River Ecology LaboratoryUniversity of GeorgiaAikenUSA
  3. 3.Department of Environmental Health ScienceUniversity of GeorgiaAthensUSA
  4. 4.Department of Ecology and Evolutionary BiologyUniversity of CaliforniaLos AngelesUSA
  5. 5.University of Alaska MuseumFairbanksUSA

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