Development and characterization of microsatellite loci for two species of Beringian birds, rock sandpiper (Calidris ptilocnemis) and Pacific wren (Troglodytes pacificus)
- 151 Downloads
Identification and assessment of small, endemic populations are priorities for conservation. We isolated and characterized 8 microsatellite loci from rock sandpiper (Calidris ptilocnemis) and 5 microsatellite loci from Pacific wren (Troglodytes pacificus), species with endemic populations of named subspecies that are of conservation concern. Eighteen to 20 individuals of each species from several locations in Alaska were screened for polymorphism. Loci for each species showed high polymorphism, with rock sandpiper ranging from 5 to 14 alleles per locus and 0.73–0.88 expected heterozygosity and Pacific wren ranging from 5 to 14 alleles per locus and 0.55–0.91 expected heterozygosity. Loci developed for rock sandpipers were also polymorphic in closely related taxa. These loci are the first developed for either species and will be used to identify and conserve endemic populations in the Bering Sea region.
KeywordsTroglodytes Calidris PCR primers Microsatellite Aleutian Islands Pribilof Islands
This work was supported by the Florida Institute of Technology, University of Alaska Museum, and by the U. S. Department of Energy under Award Number DE--FC09--07SR22506 to the University of Georgia Research Foundation. We thank M. Zimmerman, S. Garcia, M. Smith, and R. Selvam for help in the laboratory.
- Excoffier L, Laval G, Schneider S (2005) Arlequin ver. 3.0: an integrated software package for population genetics data analysis. Evol Bioinforma Online 1:47–50Google Scholar
- Gotthardt TA, Walton KM, Fields TL (2012) Setting priorities for Alaska’s Wildlife Action Plan. University of Alaska Anchorage, Alaska Natural Heritage ProgramGoogle Scholar
- Hauswaldt JS, Glenn TC (2003) Microsatellite DNA loci for the diamondback terrapin (Malaclemys terrapin). Mol Ecol Resour 3:174–176Google Scholar