Conservation Genetics Resources

, Volume 6, Issue 1, pp 59–61

Development of nineteen polymorphic microsatellite loci in the threatened polar bear (Ursus maritimus) using next generation sequencing

  • Jessica R. Brandt
  • Peter J. Van Coeverden de Groot
  • Kai Zhao
  • Markus G. Dyck
  • Peter T. Boag
  • Alfred L. Roca
Microsatellite Letters

Abstract

Habitat loss caused by the reduction of Arctic ice threatens polar bear (Ursus maritimus) populations. To aid in polar bear conservation, we used stringent marker design criteria with next generation shotgun sequencing to design primers for 19 novel polymorphic microsatellite loci. The number of alleles per locus ranged from 2 to 7, and the average observed heterozygosity across all loci was HO = 0.67. No linkage disequilibrium was detected between loci and only one locus deviated from Hardy–Weinberg equilibrium. Since genetic studies of wildlife species often rely on non-invasive fecal sampling, the microsatellite markers were designed to amplify short regions (<200 bp) to maximize the potential genotyping success when using low quality DNA. These novel markers will be useful for population and conservation genetic studies of polar bear populations.

Keywords

Conservation Next generation shotgun pyrosequencing Non-invasive methods 

Supplementary material

12686_2013_3_MOESM1_ESM.doc (208 kb)
Supplementary material (DOC 209 kb)

References

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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Jessica R. Brandt
    • 1
  • Peter J. Van Coeverden de Groot
    • 2
  • Kai Zhao
    • 1
  • Markus G. Dyck
    • 3
  • Peter T. Boag
    • 2
  • Alfred L. Roca
    • 1
  1. 1.Department of Animal SciencesUniversity of Illinois at Urbana-ChampaignUrbanaUSA
  2. 2.Department of BiologyQueen’s UniversityKingstonCanada
  3. 3.Department of EnvironmentGovernment of NunavutIgloolikCanada

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