Characterization of EST-based SSR loci in the spruce budworm, Choristoneura fumiferana (Lepidoptera: Tortricidae)
- 146 Downloads
After identifying 114 microsatellite loci from Choristoneura fumiferana expressed sequence tags, 87 loci were assayed in a panel of 11 wild-caught individuals, giving 29 polymorphic loci. Further analysis of 20 of these loci on 31 individuals collected from a single population in northern Minnesota identified 14 in Hardy–Weinberg equilibrium.
KeywordsSpruce budworm Expressed sequence tag Microsatellite Simple sequence repeat Choristoneura fumiferana Tortricidae
We thank P. Stothard for assistance in post-processing and analysis of ESTs, and P. Beldade and A. Long for the use of their SSR-mining Perl script. This study was funded by a Discovery Grant from the Natural Sciences and Engineering Research Council of Canada to F. Sperling, a Genomics Research and Development Initiative Grant (NRCAN) to D. Doucet, and a United States Department of Agriculture, Cooperative State Research Education and Extension Service, National Research Initiative Grant (WISR-2005-02815) to Brian Sturtevant.
- Boulanger Y, Arsenault D, Morin H, Jardon Y, Bertrand P, Dagneau C (2012) Dendrochronological reconstruction of spruce budworm (Choristoneura fumiferana) outbreaks in southern Quebec for the last 400 years. Can J Forest Res 42:1264–1276Google Scholar
- Ewing B, Hillier L, Wendl M, Green P (2008) Base-calling of automated sequencer traces using Phred. I. Accuracy assessment. Genome Res 8:175–185Google Scholar
- Lumley LM, Davis CS, Sperling FAH (2009) Isolation and characterization of eight microsatellite loci in the spruce budworm species Choristoneura fumiferana and Choristoneura occidentalis, and cross-species amplification in related tortricid moths. Conserv Genet Resour 1:501–504CrossRefGoogle Scholar
- Park SDE (2001) Trypanotolerance in West African cattle and the population genetic effects of selection. Ph.D. thesis, University of DublinGoogle Scholar
- Raymond M, Rousset F (1995) GENEPOP (version 1.2): population genetics software for exact tests and ecumenicism. J Hered 86:248–249Google Scholar
- Rozen S, Skaletsky HJ (2000) Primer3 on the WWW for general users and for biologist programmers. In: Krawetz S, Misener S (eds) Bioinformatics methods and protocols: methods in molecular biology. Humana Press, Totowa, pp 365–386Google Scholar
- Sinama M, Dubut V, Costedoat C, Gilles A, Junker M, Malausa T, Martin J-F, Neve G, Pech N, Schmitt T, Zimmermann M, Meglecz E (2011) Challenges of microsatellite development in Lepidoptera: Euphydryas aurinia (Nymphalidae) as a case study. Eur J Entomol 108:261–266Google Scholar
- Volney WJA, Fleming RA (2000) Climate change and impacts of boreal forest insects. Agr Ecosyst Environ 82:283–294Google Scholar