Conservation Genetics Resources

, Volume 5, Issue 2, pp 541–544 | Cite as

Characterization of EST-based SSR loci in the spruce budworm, Choristoneura fumiferana (Lepidoptera: Tortricidae)

  • B. M. T. BrunetEmail author
  • D. Doucet
  • B. R. Sturtevant
  • F. A. H. Sperling
Technical Note


After identifying 114 microsatellite loci from Choristoneura fumiferana expressed sequence tags, 87 loci were assayed in a panel of 11 wild-caught individuals, giving 29 polymorphic loci. Further analysis of 20 of these loci on 31 individuals collected from a single population in northern Minnesota identified 14 in Hardy–Weinberg equilibrium.


Spruce budworm Expressed sequence tag Microsatellite Simple sequence repeat Choristoneura fumiferana Tortricidae 



We thank P. Stothard for assistance in post-processing and analysis of ESTs, and P. Beldade and A. Long for the use of their SSR-mining Perl script. This study was funded by a Discovery Grant from the Natural Sciences and Engineering Research Council of Canada to F. Sperling, a Genomics Research and Development Initiative Grant (NRCAN) to D. Doucet, and a United States Department of Agriculture, Cooperative State Research Education and Extension Service, National Research Initiative Grant (WISR-2005-02815) to Brian Sturtevant.

Supplementary material

12686_2012_9847_MOESM1_ESM.xls (146 kb)
Online Resource 1: Characteristics of 87 EST-SSR loci tested on a validation panel of 11 individuals from Alberta. (XLS 146 kb)


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Copyright information

© © Her Majesty the Queen in Right of Canada 2013

Authors and Affiliations

  • B. M. T. Brunet
    • 1
    Email author
  • D. Doucet
    • 2
  • B. R. Sturtevant
    • 3
  • F. A. H. Sperling
    • 1
  1. 1.Department of Biological SciencesUniversity of AlbertaEdmontonCanada
  2. 2.Natural Resources Canada, Great Lakes Forestry CentreSault Ste. MarieCanada
  3. 3.USDA Forest Service, Northcentral Research StationRhinelanderUSA

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