Conservation Genetics Resources

, Volume 5, Issue 1, pp 243–249 | Cite as

Development and cross-amplification of thirty microsatellite loci in five diploid and polyploid Central Asian species of Palearctic green toads (Bufo viridis subgroup)

  • Caroline Betto-Colliard
  • Roberto Sermier
  • Nicolas Perrin
  • Matthias Stöck
Technical Note

Abstract

We report 30 polymorphic microsatellite markers for five species of Palearctic green toads (Bufo viridis subgroup): 23 in the diploid B. latastii, 19 in diploid B. turanensis, 20 in diploid B. shaartusiensis, 27 in tetraploid B. pewzowi, and 30 in triploid B. baturae. Genetic diversity at these loci, measured for 10–18 individuals per species, ranged from 2 to 19 alleles. These microsatellite loci will be useful for conservation plans (genetic diversity, population structure, evolutionary units), inheritance patterns, and evolution of green toads.

Keywords

Bufo viridis subgroup Microsatellite markers Polyploidy Hybrid speciation 

References

  1. Broquet T, Berset-Braendli L, Emaresi G, Fumagalli L (2007) Buccal swabs allow efficient and reliable microsatellite genotyping in amphibians. Conserv Genet 8:509–511CrossRefGoogle Scholar
  2. Colliard C, Sicilia A, Moritz C, Perrin N, Stöck M (2009) New polymorphic microsatellite markers and development of mitotyping primers for West Mediterranean green toad species (Bufo viridis subgroup). Mol Ecol Resour 9:1138–1140PubMedCrossRefGoogle Scholar
  3. Colliard C, Sicilia A, Turrisi GF, Arculeo M, Perrin N, Stöck M (2010) Strong reproductive barriers in a narrow hybrid zone of West-Mediterranean green toads (Bufo viridis subgroup) with Plio-Pleistocene divergence. BMC Evol Biol 10:232PubMedCrossRefGoogle Scholar
  4. Dufresnes C, Betto-Colliard C, Perrin N, Stöck M (2010) Thirteen polymorphic microsatellite markers for the European green toad Bufo viridis viridis, a declining amphibian species. Conserv Genet Resour 3:311–313CrossRefGoogle Scholar
  5. Evans BJ, Pyron RA, Wiens JJ (2012) Polyploidization and sex chromosome evolution in amphibians. In: Soltis PS, Soltis DE (eds) Polyploidy and genome evolution, chap 18. Springer, Heidelberg (in press)Google Scholar
  6. Ficetola GF, Crottini A, Casiraghi M, Padoa-Schioppa E (2010) New data on amphibians and reptiles of the Northern Areas of Pakistan: distribution, genetic variability and conservation issues. North-West J Zool 6:1–12Google Scholar
  7. Goudet J (1995) FSTAT (Version1.2): a computer program to calculate F-statistics. J Hered 86:485–486Google Scholar
  8. Kawamura T (1984) Polyploidy in amphibians. Zool Sci 1984(1):1–15Google Scholar
  9. Mable B (2004) ‘Why polyploidy is rarer in animals than in plants’: myths and mechanisms. Biol J Linn Soc 82:453–466CrossRefGoogle Scholar
  10. Mable BK, Alexandrou MA, Taylor MI (2011) Genome duplication in amphibians and fish: an extended synthesis. J Zool 284:151–182CrossRefGoogle Scholar
  11. Stöck M, Lamatsch DK, Steinlein C, Epplen JT, Grosse R, Hock R, Klapperstück KP, Lampert U, Scheer M, Schmid M, Scartl M (2002) A bisexually reproducing all-triploid vertebrate. Nat Genet 30(3):325–328PubMedCrossRefGoogle Scholar
  12. Stöck M, Moritz C, Hickerson M, Frynta D, Dujsebayeva T, Eremchenko V, Robert Macey J, Papenfuss TJ, Wake DB (2006) Evolution of mitochondrial relationships and biogeography of Palearctic green toads (Bufo viridis subgroup) with insights in their genomic plasticity. Mol Phylogenet Evol 41:663–689PubMedCrossRefGoogle Scholar
  13. Stöck M, Ustinova J, Lamatsch DK, Schartl M, Perrin N, Moritz C (2010) A vertebrate reproductive system involving three ploidy levels: hybrid origin of triploids in a contact zone od diploid and tetraploid paleartic green toads (Bufo viridis subgroup). Evolution 64(4):944–959PubMedCrossRefGoogle Scholar
  14. Stöck M, Ustinova J, Betto-Colliard C, Schartl M, Moritz C, Perrin N (2012) Simultaneous Mendelian and clonal genome transmission in an all-triploid vertebrate. Proc R Soc B 279(1732):1293–1299PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2012

Authors and Affiliations

  • Caroline Betto-Colliard
    • 1
  • Roberto Sermier
    • 1
  • Nicolas Perrin
    • 1
  • Matthias Stöck
    • 2
  1. 1.Department of Ecology and EvolutionUniversity of LausanneLausanneSwitzerland
  2. 2.Leibniz-Institute of Freshwater Ecology and Inland Fisheries (IGB)BerlinGermany

Personalised recommendations