Twenty-two novel microsatellite loci for lumpfish (Cyclopterus lumpus)
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Lumpfish (Cyclopterus lumpus L. 1758) are widely distributed on both sides of the North Atlantic. They are a commercially important species, but stock size estimates have declined since the mid-1980s in Canada, Norway and Iceland. Little is known about the biology of this species, in particular the breeding migrations and population structure which are fundamental for effective management. This paper describes the development and characterization of twenty-two polymorphic microsatellite loci using next generation sequencing. The number of alleles per locus ranged from 3 to 27 in two geographically distant North Atlantic populations, with observed and expected heterozygosities ranging between 0.0625–0.979 and 0.0618–0.946, respectively. These loci are an important resource that will allow assessment of the population genetic structure of this species, and contribute to its appropriate management.
KeywordsLumpfish Microsatellite Genotyping Next generation sequencing
The authors thank Monty Way and Alain Fréchet for providing the Canadian samples and Halldór Gunnar Ólafsson at BioPol and the Icelandic lumpfish harvesters for their cooperation and support in our sampling efforts. We thank Alexandra M. Klonowski, Ágústa Sigfúsdóttir, Steinunn Magnúsdóttir and Snædís Björnsdóttir for their assistance in the laboratory. This research was supported by The Fisheries Research Fund of the Icelandic Ministry of Fisheries and Agriculture.
- Anon (2011) The state of marine stocks in Icelandic waters 2010/2011—prospects for the quota year 2011/2012. Hafrannsoknir 159:172Google Scholar
- Brownstein MJ, Carpten JD, Smith JR (1996) Modulation of non-templated nucleotide addition by Taq DNA polymerase: primer modifications that facilitate genotyping. Biotechniques 20(1004–1006):1008–1010Google Scholar
- Davenport J (1985) Synopsis of biological data on the lumpsucker Cyclopterus lumpus (Linnaeus, 1758). FAO Fisheries Synopsis No. 147Google Scholar
- Drummond AJ, Ashton B, Buxton S, Cheung M et al (2011) Geneious v5.6. Available from www.geneious.com
- Raymond M, Rousset F (1995) GENEPOP (Version 1.2): population genetics software for exact tests and ecumenicism. J Hered 86:248–249Google Scholar
- Schopka SA (1974) Preliminary results from tagging of lumpsucker (Cyclopterus lumpus), in Icelandic waters 1971–1973. ICES CM 1974:F18Google Scholar
- Sunnana K (2007) Rognkjeks. Kyst og havbruk 2007. Fisken og havet 2–2007:86–87Google Scholar
- Thorsteinsson V (1981) The ageing validation of the lumpsucker (Cyclopterus lumpus) and the age composition of the lumpsucker in the Icelandic lumpsucker fisheries. ICES CM 1981:G58Google Scholar