Conservation Genetics Resources

, Volume 5, Issue 1, pp 173–175 | Cite as

Isolation and characterization of microsatellite loci from Iguana delicatissima (Reptilia: Iguanidae), new perspectives for investigation of hybridization events with Iguana iguana

  • V. Valette
  • L. Filipová
  • B. Vuillaume
  • C. Cherbonnel
  • A. M. Risterucci
  • C. Delaunay
  • M. Breuil
  • F. Grandjean
Technical Note
First Online:
Received:
Accepted:

Abstract

A (GA)n and (GT)n microsatellite-enriched library was constructed and 25 nuclear simple sequence repeat (SSR) loci were characterized in the Lesser Antillean Iguana (Iguana delicatissima). All SSR loci were found to be polymorphic after screening for diversity in different cultivars, and a cross-taxa amplification tests showed the potential transferability of most SSR markers in Iguana iguana. First to be published for I. delicatissima, this new SSR resource will be a powerful tool for intraspecific genetic studies and for investigation of hybridization events with Iguana iguana.

Keywords

Iguana delicatissima Iguana iguana Microsatellite 
This is a preview of subscription content, log in to check access.

References

  1. Billote N, Lagoda PJL, Risterucci AM, Baurens FC (1999) Microsatellite-enriched libraries: applied methodology for the development of SSR markers in tropical crops. Fruits 54(4):277–288Google Scholar
  2. Breuil M (2002) Histoire naturelle des Amphibiens et Reptiles terrestres de l’Archipel Guadeloupéen. Guadeloupe et dépendances, Saint-Martin, Saint-Barthélemy. Patrimoines naturels, IEGB, SPN MNHN, 54:339.Google Scholar
  3. Breuil M, Day M, Knapp C (2010) Iguana delicatissima. In: IUCN 2010. IUCN Red List of Threatened Species. http://www.iucnredlist.org
  4. Roy MS, Gefen E, Smith JD, Ostrander EA, Wayne RK (1994) Mol Biol Evol 11(4):553–570PubMedGoogle Scholar
  5. Rozen S, Skaletsky HJ (2000) Primer3 on the WWW for general users and for biologist programmers. In: Krawetz S, Misener S (eds) Bioinformatics methods and protocols: methods in molecular biology. Humana Press, Totowa, pp 365–386. Source code available at http://fokker.wi.mit.edu/primer3/

Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • V. Valette
    • 1
  • L. Filipová
    • 2
  • B. Vuillaume
    • 4
  • C. Cherbonnel
    • 1
  • A. M. Risterucci
    • 3
  • C. Delaunay
    • 2
  • M. Breuil
    • 4
  • F. Grandjean
    • 2
  1. 1.GenindexeLa RochelleFrance
  2. 2.Laboratoire Ecologie, Evolution, Symbiose, UMR CNRS 6556Université de PoitiersPoitiers CedexFrance
  3. 3.CIRAD (Centre de coopération Internationale en Recherche Agronomique pour le Développement), UMR 108 AGAPMontpellier Cedex 5France
  4. 4.Département de Systématique et d’Évolution, Amphibiens-ReptilesMuséum national d’Histoire naturelleParis Cedex 05France

Personalised recommendations