Conservation Genetics Resources

, Volume 5, Issue 1, pp 145–148

Development and characterization of twenty-three microsatellite markers for the freshwater minnow Santa Ana speckled dace (Rhinichthys osculus spp., Cyprinidae) using paired-end Illumina shotgun sequencing

  • Schyler O. Nunziata
  • Stacey L. Lance
  • Kenneth L. Jones
  • Stacey A. Nerkowski
  • Anthony E. Metcalf
Technical Note

DOI: 10.1007/s12686-012-9754-y

Cite this article as:
Nunziata, S.O., Lance, S.L., Jones, K.L. et al. Conservation Genet Resour (2013) 5: 145. doi:10.1007/s12686-012-9754-y

Abstract

We isolated and characterized a total of 23 microsatellite loci from the Santa Ana speckled dace, Rhinichthys osculus spp., a freshwater minnow restricted to southern California. Loci were screened in 24 individuals from five watersheds. The number of alleles per locus ranged from 7 to 25, observed heterozygosity ranged from 0.409 to 0.875, and the probability of identity values ranged from 0.005 to 0.081. These new loci will provide tools for examining taxonomic status, population structure, and management strategies.

Keywords

Rhinichthys osculus Illumina Microsatellite PAL_Finder PCR primers SSR STR 

Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • Schyler O. Nunziata
    • 1
  • Stacey L. Lance
    • 1
  • Kenneth L. Jones
    • 2
  • Stacey A. Nerkowski
    • 3
  • Anthony E. Metcalf
    • 3
  1. 1.Savannah River Ecology LaboratoryUniversity of GeorgiaAikenUSA
  2. 2.Department of Biochemistry and Molecular Genetics, School of MedicineUniversity of ColoradoAuroraUSA
  3. 3.Department of BiologyCalifornia State University, San BernardinoSan BernardinoUSA

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