Characterisation and cross-amplification of fourteen microsatellite loci for the endemic New Zealand tui (Meliphagidae), Prosthemadera novaeseelandiae
- 169 Downloads
We describe eight novel microsatellite loci for the tui, Prosthemadera novaeseelandiae, and the cross-amplification of six polymorphic loci developed for the bellbird (Anthornis melanura). Fifty tui samples from Tawharanui Regional Park, New Zealand, were genotyped using the 14 loci described. Numbers of alleles ranged from 3 to 16. Observed and expected heterozygosities ranged between 0.180–0.940 and 0.237–0.878, respectively. There was no evidence of linkage disequilibrium. One locus (Ame-22) significantly deviated from Hardy–Weinberg Equilibrium. This study highlights the benefits of long-repeat microsatellites in minimising scoring errors. These are the first microsatellites to be developed for the tui and also provide potential markers for studying other honeyeaters throughout the Austro-Papuan region.
KeywordsMicrosatellite Tui Prosthemadera novaeseelandiae Honeyeater Meliphagidae Bellbird Anthornis melanura New Zealand
We thank the University of Otago High-Throughput Sequencing Unit for use of their next-generation sequencing facility which produced the sequence reads for the development of the microsatellites. We thank Fatima Torrico, Helen Cadwaller, Ulla Kail, Sam Hill, and Damien Menard for their help in the field, and Robyn Howitt, Ana Ramón-Laca, and Diana Prada for advice in the lab. This work was supported by Massey University’s Institute of Natural Sciences, Landcare Research Ltd, and the Auckland Council.
- Hedrick PW (2009) Genetics of populations, 4th edn. Jones and Bartlett, SudburyGoogle Scholar
- Margulies M, Egholm M, Altman WE, Attiya S, Bader JS, Bemben LA, Berka J, Braverman MS, Chen YJ, Chen Z, Dewell SB, Du L, Fierro JM, Gomes XV, Godwin BC, He W, Helgesen S, Ho CH, Irzyk GP, Jando SC, Alenquer ML, Jarvie TP, Jirage KB, Kim JB, Knight JR, Lanza JR, Leamon JH, Lefkowitz SM, Lei M, Li J, Lohman KL, Lu H, Makhijani VB, McDade KE, McKenna MP, Myers EW, Nickerson E, Nobile JR, Plant R, Puc BP, Ronan MT, Roth GT, Sarkis GJ, Simons JF, Simpson JW, Srinivasan M, Tartaro KR, Tomasz A, Vogt KA, Volkmer GA, Wang SH, Wang Y, Weiner MP, Yu P, Begley RF, Rothberg JM (2005) Genome sequencing in microfabricated high-density picolitre reactors. Nature 437(7057):376–380PubMedGoogle Scholar
- Rozen S, Skaletsky HJ (2000) Primer3 on the WWW for general users and for biologist programmers. In: Krawetz S, Misener S, Krawetz S (eds) Miseners. Bioinformatics methods and protocols: methods in molecular biology. Humana Press, Totowa, pp 365–386Google Scholar