Conservation Genetics Resources

, Volume 5, Issue 1, pp 113–116 | Cite as

Characterisation and cross-amplification of fourteen microsatellite loci for the endemic New Zealand tui (Meliphagidae), Prosthemadera novaeseelandiae

  • S. J. WellsEmail author
  • W. Ji
  • S. M. Baillie
  • D. Gleeson
Technical Note


We describe eight novel microsatellite loci for the tui, Prosthemadera novaeseelandiae, and the cross-amplification of six polymorphic loci developed for the bellbird (Anthornis melanura). Fifty tui samples from Tawharanui Regional Park, New Zealand, were genotyped using the 14 loci described. Numbers of alleles ranged from 3 to 16. Observed and expected heterozygosities ranged between 0.180–0.940 and 0.237–0.878, respectively. There was no evidence of linkage disequilibrium. One locus (Ame-22) significantly deviated from Hardy–Weinberg Equilibrium. This study highlights the benefits of long-repeat microsatellites in minimising scoring errors. These are the first microsatellites to be developed for the tui and also provide potential markers for studying other honeyeaters throughout the Austro-Papuan region.


Microsatellite Tui Prosthemadera novaeseelandiae Honeyeater Meliphagidae Bellbird Anthornis melanura New Zealand 



We thank the University of Otago High-Throughput Sequencing Unit for use of their next-generation sequencing facility which produced the sequence reads for the development of the microsatellites. We thank Fatima Torrico, Helen Cadwaller, Ulla Kail, Sam Hill, and Damien Menard for their help in the field, and Robyn Howitt, Ana Ramón-Laca, and Diana Prada for advice in the lab. This work was supported by Massey University’s Institute of Natural Sciences, Landcare Research Ltd, and the Auckland Council.


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Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • S. J. Wells
    • 1
    Email author
  • W. Ji
    • 2
  • S. M. Baillie
    • 3
  • D. Gleeson
    • 4
    • 5
  1. 1.Ecology and Conservation Group, Institute of Natural SciencesMassey UniversityAucklandNew Zealand
  2. 2.Ecology and Conservation Group, Institute of Natural SciencesMassey UniversityAucklandNew Zealand
  3. 3.Ocean Tracking Network Canada, Department of Biology, Life Science CentreDalhousie UniversityHalifaxCanada
  4. 4.Institute of Applied EcologyUniversity of CanberraCanberraAustralia
  5. 5.EcoGeneLandcare Research Ltd.AucklandNew Zealand

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