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Conservation Genetics Resources

, Volume 5, Issue 1, pp 69–72 | Cite as

Microsatellite loci development for rare Colorado Sclerocactus (Cactaceae)

  • Anna L. Schwabe
  • Ashley R. Hubbard
  • Jennifer Ramp Neale
  • Mitchell E. McGlaughlin
Technical Note

Abstract

The genus Sclerocactus (Cactaceae) consists of 15 species, which have a confused taxonomic history due to morphological similarities and distribution overlap. Habitat loss and hybridization are of concern and have established cause for genetic investigations to further understand the genus and develop conservation strategies. Thirteen variable microsatellite loci were identified using S. glaucus and S. parviflorus and were tested in three additional species: S. wetlandicus, S. brevispinus and S. cloverii. The mean number of alleles per locus ranged from 2 to 11, with an average of 6.6. The observed and expected mean heterozygosity ranged from 0.00 to 0.90 and 0.26 to 0.90, respectively. These loci will aid in determining levels of hybridization, diversity and taxonomy of Sclerocactus.

Keywords

Colorado Conservation genetics Sclerocactus Microsatellite 

Notes

Acknowledgments

This research was funded by the United States Fish and Wildlife Service. Floral tissue was collected by Denver Botanic Gardens and laboratory research was conducted at the University of Northern Colorado.

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Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • Anna L. Schwabe
    • 1
  • Ashley R. Hubbard
    • 1
  • Jennifer Ramp Neale
    • 2
  • Mitchell E. McGlaughlin
    • 1
  1. 1.School of Biological SciencesUniversity of Northern ColoradoGreeleyUSA
  2. 2.Denver Botanic GardensDenverUSA

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