Microsatellite loci development for rare Colorado Sclerocactus (Cactaceae)
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The genus Sclerocactus (Cactaceae) consists of 15 species, which have a confused taxonomic history due to morphological similarities and distribution overlap. Habitat loss and hybridization are of concern and have established cause for genetic investigations to further understand the genus and develop conservation strategies. Thirteen variable microsatellite loci were identified using S. glaucus and S. parviflorus and were tested in three additional species: S. wetlandicus, S. brevispinus and S. cloverii. The mean number of alleles per locus ranged from 2 to 11, with an average of 6.6. The observed and expected mean heterozygosity ranged from 0.00 to 0.90 and 0.26 to 0.90, respectively. These loci will aid in determining levels of hybridization, diversity and taxonomy of Sclerocactus.
KeywordsColorado Conservation genetics Sclerocactus Microsatellite
This research was funded by the United States Fish and Wildlife Service. Floral tissue was collected by Denver Botanic Gardens and laboratory research was conducted at the University of Northern Colorado.
- Glenn TC (2006) 5′ Primer tags; a cheap way to flourecently tag PCR products. Unpublished manual. Savannah River Ecology Lab, Department of Biological Sciences. University of South Carolina, ColumbiaGoogle Scholar
- Glenn TC, Schable M (2002) Microsatellite isolation with dynabeads 2002. Unpublished manual. Savannah River Ecology Laboratory, Aiken, South CarolinaGoogle Scholar
- Heil KD, Porter JM (2004) Sclerocactus. In: Flora of North America Editorial Committee (ed) Flora of north America, north of Mexico, vol 4. Magnoliophyta: Caryophyllidae, part 1. Oxford University Press, NY, pp 197–207Google Scholar
- Hochstätter F (1989) Nieubeschrijving Sclerocactus wetlandicus species nova-Een nieuwe Sclerocactus uit Uintah County, Utah, USA. Succulenta 68:123–126Google Scholar
- Porter JM, Kinnery MS, Heil KD (2000) Relationships between Sclerocactus and Toumeya (Cactaceae) based on chloroplast trnL-trnF sequences. Haseltonia 7:8–23Google Scholar
- Raymond M, Rousset F (1995) GENEPOP (version 1.2): population genetics software for exact tests and ecumenicism. J Heredity 86:248–249Google Scholar
- Rozen S, Skaletsky HJ (2000) Primer3 on the WWW for general users and for biologist programmers. In: Misener S, Krawetz SA (eds) Bioinformatics methods and protocols. Humana Press, Totowa, pp 365–386Google Scholar
- USDA (2011) Technical note: threatened, endangered, candidate and proposed plant species of Utah. TN Plant Mater 52:42–43Google Scholar
- USFWS (1979) Determination that Sclerocactus glaucus is a threatened species. Fed Reg 44:58868–58870Google Scholar