Conservation Genetics Resources

, Volume 5, Issue 1, pp 23–26

Tetranucleotide microsatellite loci from the critically endangered hawksbill turtle (Eretmochelys imbricata)

  • Brian M. Shamblin
  • Bonnie E. Berry
  • Denise M. Lennon
  • Anne B. Meylan
  • Peter A. Meylan
  • Mark E. Outerbridge
  • Campbell J. Nairn
Technical Note
  • 183 Downloads

Abstract

We describe isolation and characterization of 14 polymorphic tetranucleotide loci from the hawksbill turtle (Eretmochelys imbricata). We identified an average of 14.5 alleles per locus based on screening of 36 individuals captured on foraging grounds in Bocas del Toro Province, Panama, and 9.6 alleles in 13 individuals captured on foraging grounds in Bermuda. Observed heterozygosity ranged from 0.67 to 1.00, with a mean of 0.85 for the Panama foraging aggregation and 0.83 for the Bermuda foraging aggregation. This microsatellite suite has a combined non-exclusion probability of identity of 8.26 × 10–23. These markers should be informative in individual and population-focused analyses.

Keywords

Eretmochelys imbricata Hawksbill turtle Microsatellite Population 

References

  1. Alstad TI, Shamblin BM, Bagley DA, Ehrhart LM, Nairn CJ (2011) Isolation and characterization of tetranucleotide microsatellites from the leatherback turtle (Dermochelys coriacea). Conserv Genet Resour 1:213–215Google Scholar
  2. Boutin-Ganache I, Raposo M, Raymond R, Deschepper CF (2001) M13-tailed primers improve the readability and usability of microsatellite analyses performed with two different allele sizing methods. Biotechniques 31:24–28Google Scholar
  3. Don RH, Cox PT, Wainwright BJ, Baker K, Mattick JS (1991) ‘Touchdown PCR to circumvent spurious priming during gene amplification. Nucleic Acids Res 19:4008PubMedCrossRefGoogle Scholar
  4. Faircloth BC (2006) GMCONVERT: file conversion for GENEMAPPER output files. Mol Ecol Notes 6:968–970CrossRefGoogle Scholar
  5. Faircloth B (2008) MSTACOMMANDER: detection of microsatellite repeat arrays and automated, locus-specific primer design. Mol Ecol Resour 8:92–94PubMedCrossRefGoogle Scholar
  6. FitzSimmons NN, Moritz C, Moore SS (1995) Conservation and dynamics of microsatellite loci over 300 million years of marine turtle evolution. Mol. Biol Evol 12:432–440PubMedGoogle Scholar
  7. Glenn TC, Schable NA (2005) Isolating microsatellite DNA loci. In: Zimmer EA, Roalson EH (eds) Methods in enzymology 395, molecular evolution: producing the biochemical data, part B. Academic Press, San DiegoGoogle Scholar
  8. Kalinowski ST, Taper ML, Marshall TC (2007) Revising how the computer program CERVUS accommodates genotyping error increases success in paternity assignment. Mol Ecol 16:1099–1106PubMedCrossRefGoogle Scholar
  9. Lin G, Chang A, Yap HW, Yue GH (2008) Characterization and cross-species amplification of microsatellites from the endangered hawksbill turtle (Eretmochelys imbricata). Conserv Genet 9:1071–1073CrossRefGoogle Scholar
  10. McClenachan L, Jackson JBC, Newman MJH (2006) Conservation implications of historic sea turtle nesting beach loss. Front Ecol Environ 4:290–296CrossRefGoogle Scholar
  11. Meylan AB (1999) Status of the hawksbill turtle (Eretmochelys imbricata) in the Caribbean region. Chelonian Conserv Biol 3:177–184Google Scholar
  12. Miro-Herrans AT, Velez-Zuazo X, Acevedo JP, McMillan WO (2008) Isolation and characterization of novel microsatellites from the critically endangered hawksbill sea turtle (Eretmochelys imbricata). Mol Ecol Resour 8:1098–1101PubMedCrossRefGoogle Scholar
  13. Parsons JJ (1972) The hawksbill turtle and the tortoise shell trade. In: Études de Géographie Tropicale Offertes á Pierre Gourou. Mouton, Sorbonne, pp 45–60Google Scholar
  14. Raymond M, Rousset F (1995) An exact test for population differentiation. Evolution 49:1280–1283Google Scholar
  15. Rosen S, Skaletsky H (2000) Primer3 on the WWW for general users and for biologist programmers. In: Krawetz S, Misener S (eds) Bioinformatics methods and protocols: methods in molecular biology. Humana Press, Totowa, pp 365–386Google Scholar
  16. Shamblin BM, Berry BE, Lennon DM, Bagley DA, Ehrhart LM, Nairn CJ (2012) Tetranucleotide microsatellite loci from the endangered green turtle (Chelonia mydas). Conserv Genet Resour. doi:10.1007/s12686-012-9643-4
  17. Shamblin BM, Faircloth BC, Dodd M, Wood-Jones A, Castleberry SB, Carroll JP, Nairn CJ (2007) Tetranculeotide microsatellites from the loggerhead sea turtle (Caretta caretta). Mol Ecol Notes 7:784–787CrossRefGoogle Scholar
  18. Shamblin BM, Faircloth BC, Dodd MG, Bagley DA, Ehrhart LM, Dutton PH, Frey A, Nairn CJ (2009) Tetranucleotide microsatellites from the loggerhead sea turtle (Caretta caretta) and their cross-amplification in other marine turtle species. Conserv Genet 10:577–580CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • Brian M. Shamblin
    • 1
  • Bonnie E. Berry
    • 1
  • Denise M. Lennon
    • 1
  • Anne B. Meylan
    • 2
  • Peter A. Meylan
    • 3
  • Mark E. Outerbridge
    • 4
  • Campbell J. Nairn
    • 1
  1. 1.Daniel B. Warnell School of Forestry and Natural ResourcesUniversity of GeorgiaAthensUSA
  2. 2.Fish and Wildlife Research InstituteFlorida Fish and Wildlife Conservation CommissionSt. PetersburgUSA
  3. 3.Natural Sciences, Eckerd CollegeSt. PetersburgUSA
  4. 4.Bermuda Turtle ProjectBermuda Zoological SocietyFlattsBermuda

Personalised recommendations