Conservation Genetics Resources

, Volume 4, Issue 4, pp 889–891

Isolation of microsatellite loci in the Amazon sailfin catfish Pterygoplichlhys pardalis (Castelneau, 1855) (Teleostei: Loricariidae)

  • Rosalina P. Pereira
  • Carlos Henrique dos A. dos Santos
  • Priscila Roberta M. Nascimento
  • Gisele T. Clímaco
  • Adna Cristina B. Sousa
  • Tatiana Campos
  • Alexandre Manoel K. Vergueiro-Júnior
  • Maria N. Paula-Silva
  • Vera Maria F. Almeida-Val
Technical Note

DOI: 10.1007/s12686-012-9666-x

Cite this article as:
Pereira, R.P., dos Santos, C.H.A., Nascimento, P.R.M. et al. Conservation Genet Resour (2012) 4: 889. doi:10.1007/s12686-012-9666-x
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Abstract

Pterygoplichlhys pardalis is an important freshwater ornamental fish in the Amazon Basin. Studies involving populations of P. pardalis are of great importance for the conservation and management of this species. We developed nine microsatellite loci and applied them to investigate the genetic variation of 20 wild individuals from floodplain lakes of the Solimões river. The number of alleles per locus ranged from 2 to 12, with an average of 6.6. The observed and expected heterozygosity values ranged from 0.400 to 0.923 (average 0.706) and from 0.358 to 0.895 (average 0.692), respectively. The value of f ranged from −0.532 to 0.467 (average 0.032). One locus (Pp07) significantly deviated from Hardy–Weinberg equilibrium after Bonferroni correction (P: (5 %) < 0.006). No significant linkage disequilibrium was detected. These microsatellite loci will contribute towards studies of genetic diversity and conservation of P. pardalis.

Keywords

Pterygoplichlhys pardalis Genetic diversity Wildlife conservation Microsatellite markers 

Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • Rosalina P. Pereira
    • 1
  • Carlos Henrique dos A. dos Santos
    • 1
  • Priscila Roberta M. Nascimento
    • 1
  • Gisele T. Clímaco
    • 1
  • Adna Cristina B. Sousa
    • 2
  • Tatiana Campos
    • 3
  • Alexandre Manoel K. Vergueiro-Júnior
    • 1
  • Maria N. Paula-Silva
    • 1
  • Vera Maria F. Almeida-Val
    • 1
  1. 1.Laboratório de Ecofisiologia e Evolução Molecular (LEEM)Instituto Nacional de Pesquisas da Amazônia (INPA)ManausBrazil
  2. 2.Universidade do Estado da BahiaPaulo AfonsoBrazil
  3. 3.Empresa Brasileira de Pesquisa AgropecuáriaRio BrancoBrazil

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