Development and characterization of polymorphic microsatellite markers of Fejervarya syhadrensis useful for genetic studies
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We describe here ten new microsatellite markers of Fejervarya syhadrensis (long-legged cricket frog). These markers were developed from the short sequence repeat-enriched genomic library and further validated using 34 frog samples collected from different parts of India. All markers were found to be polymorphic revealing 6–30 alleles in the tested samples, with the observed heterozygosity and PIC values varying from 0.067–0.882 to 0.573–0.944, respectively. These markers also showed cross-species/cross-genera transferability to varying extent when tested on four related species of Fejervarya and two species of related genera Hoplobatrachus tigerninus and Euphlyctis cyanophlyctis. Importantly, four markers Fej_ccmb_01, Fej_ccmb_04, Fej_ccmb_06, and Fej_ccmb_07 showed 100 % transferability both across species and genera. Thus, this first report of ten new microsatellite markers is expected to greatly facilitate population genetic studies aimed to understand biogeography of Fejervarya, and/or the effect of various anthropogenic factors on amphibian decline.
KeywordsFejervarya Microsatellite markers Conservation Population genetics Cross-species transferability
The authors thank the Department of Biotechnology, India for the financial support and the Council for Scientific and Industrial Research for the lab facilities; Justin, Phanindranath, Prasoona, Kiranmayi for the technical support extended for the study; Niladhari and Prudhvi for the population samples that were collected under the CCMB-WII-NOU collaborative project (DBT, Govt of India, BT/PR8354/NDB/51/141/2006) on anuran barcoding.
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