Conservation Genetics Resources

, Volume 4, Issue 3, pp 787–789

Development and characterization of sixteen microsatellite markers for the federally endangered species: Leptodea leptodon (Bivalvia: Unionidae) using paired-end Illumina shotgun sequencing

  • Jason O’Bryhim
  • Jer Pin Chong
  • Stacey L. Lance
  • Kenneth L. Jones
  • Kevin J. Roe
Technical Note

DOI: 10.1007/s12686-012-9644-3

Cite this article as:
O’Bryhim, J., Chong, J.P., Lance, S.L. et al. Conservation Genet Resour (2012) 4: 787. doi:10.1007/s12686-012-9644-3

Abstract

We isolated and characterized a total of 16 microsatellite loci in Leptodea leptodon. Loci were screened in 24 individuals from across the species current range in Missouri. The number of alleles per locus ranged from 3 to 17, observed heterozygosity ranged from 0.208 to 1.000, and the probability of identity values ranged from 0.014 to 0.67. These new loci will be used for conducting investigations into the genetic structure and diversity of extant populations of this federally endangered species.

Keywords

Leptodea Microsatellite PAL_FINDER PCR primers SSR 

Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • Jason O’Bryhim
    • 1
    • 2
  • Jer Pin Chong
    • 4
  • Stacey L. Lance
    • 1
  • Kenneth L. Jones
    • 3
  • Kevin J. Roe
    • 4
  1. 1.Savannah River Ecology LaboratoryUniversity of GeorgiaAikenUSA
  2. 2.Department of Environmental Science and PolicyGeorge Mason UniversityFairfaxUSA
  3. 3.Department of Biochemistry and Molecular GeneticsUniversity of Colorado School of MedicineAuroraUSA
  4. 4.Department of Natural Resource Ecology and ManagementIowa State UniversityAmesUSA

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