Isolation and characterization of eleven novel polymorphic microsatellite loci in the spiny softshell turtle (Apalone spinifera)
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We isolate and characterize 11 microsatellite loci for the spiny softshell turtle (Apalone spinifera) from a partial genomic library obtained using 454 sequencing technology. Genotypes of 15 individuals from southern Ontario and 30 individuals of unknown origin contain 6–20 alleles per locus and the level of heterozygosity ranges from 0.229 to 0.800. These loci will be useful for population genetics studies and enforcement activities such as assignment of illegally traded individuals to their population of origin.
KeywordsMicrosatellite Next-generation sequencing Turtle
This project was made possible by a Canada Collection grant from Wildlife Preservation Canada to C.D. and a National Science and Engineering Research Council (NSERC) Discovery Grant A3148 to R.W.M. Generous assistance from the Schad Foundation offset genotyping costs. C.D. and I.D. are funded by NSERC Canada Graduate Scholarships; D.S. was funded by an NSERC Undergraduate Summer Research Award. We thank Dr. C. Kyle, E. Kerr and M. Harnden at the NRDPFC (Trent University) for assistance with 454 sequencing. Sample collection was conducted with the permission of the Government of Ontario following Animal Use Protocol 2010–14 (Royal Ontario Museum, Toronto, Canada). We thank R. Andrews and the Lake Ontario Enforcement Unit for providing tissues from confiscated turtles.
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