Conservation Genetics Resources

, Volume 4, Issue 3, pp 737–741 | Cite as

Identification and evaluation of novel di- and tetranucleotide microsatellite markers from the brown bear (Ursus arctos)

  • Oddmund Kleven
  • Björn M. Hallström
  • Frank Hailer
  • Axel Janke
  • Snorre B. Hagen
  • Alexander Kopatz
  • Hans Geir Eiken
Technical Note

Abstract

We here describe 34 novel microsatellite loci from the brown bear (Ursus arctos) identified using a next-generation sequencing approach. While we found allelic variation in 30 of these, we selected 16 polymorphic microsatellites (11 di- and 5 tetranucleotide repeats) for four multiplex-PCR assays based on genetic variability, PCR performance and interpretability. Applying these assays for the 16 novel microsatellites, we screened 31 brown bears from a northern European population. The number of alleles encountered at single loci was between 4 and 10, and expected and observed heterozygosities ranged between 0.66–0.85 and 0.60–0.97, respectively. Seven of 120 pairwise locus combinations showed linkage disequilibrium and one locus deviated from Hardy–Weinberg equilibrium, while no null-alleles were detected. Our novel multiplex-PCR assays may be useful in forensics, parentage and population genetic studies.

Keywords

Di- and tetranucleotide microsatellites High-throughput sequencing Multiplex PCR Non-invasive samples Genetic variation Ursus arctos 

Supplementary material

12686_2012_9634_MOESM1_ESM.pdf (251 kb)
Supplementary material 1 (PDF 251 kb)
12686_2012_9634_MOESM2_ESM.pdf (48 kb)
Supplementary material 2 (PDF 47 kb)

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Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • Oddmund Kleven
    • 1
  • Björn M. Hallström
    • 2
  • Frank Hailer
    • 2
  • Axel Janke
    • 2
    • 3
  • Snorre B. Hagen
    • 1
  • Alexander Kopatz
    • 1
  • Hans Geir Eiken
    • 1
  1. 1.Bioforsk Soil and Environment, SvanhovdNorwegian Institute for Agricultural and Environmental ResearchSvanvikNorway
  2. 2.LOEWE, Biodiversity and Climate Research Centre, BiK-FSenckenberg GesellSchaft für NaturforschungFrankfurtGermany
  3. 3.Goethe University FrankfurtFrankfurtGermany

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